Recombinant protein biosensors and a method for detecting the presence of an analyte molecule
OwnerSaurabh Rajendra Nirantar, Farid John Ghadessyinventors
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Abstract
The present invention refers to a fusion protein biosensor, comprising a peptide or protein domain that binds an analyte of interest (A), an entity that can produce a detectable signal (B), and an entity that binds to B and modulate the signal produced by B when A is not bound to the analyte. A method and a kit for detecting a presence or amount of an analyte molecule using the fusion protein is also disclosed.
Claims37
A fusion protein comprising a structure of
- (An-B-Ax-C-Am)y
- wherein
- each A independently comprises a peptide or protein domain that binds an analyte of interest;
- B comprises an entity that can produce a detectable signal;
- C comprises an entity that, when A is not bound to the analyte of interest, binds to B and modulates the signal production by B;
- n and m are each independently 0 or at least 1;
- x is an integer of 0 or at least 1;
- y is an integer of at least 1;
- “-” represents a covalent bond or a linker comprising or consisting of one or more amino acids;
- provided that if x is 0, n, m or both are at least 1.
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A method for detecting a presence or amount of an analyte molecule, the method comprising:
- contacting a fusion protein comprising a structure of
- (An-B-Ax-C-Am)y
- wherein
- each A independently comprises a peptide or protein domain that binds an analyte of interest;
- B comprises an entity that can produce a detectable signal;
- C comprises an entity that, when A is not bound to the analyte of interest, binds to B and modulates the signal production by B;
- n and m are each independently 0 or at least 1;
- x is an integer of 0 or at least 1;
- y is an integer of at least 1;
- “-” represents a covalent bond or a linker comprising or consisting of one or more amino acids;
- provided that if x is 0, n, m or both are at least 1, with the analyte molecule under conditions that allow binding of A to the analyte molecule;
- and
- detecting the presence of the analyte molecule by determining a signal produced by the fusion protein:analyte complex.
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A kit for detecting a presence of an analyte molecule or an amount of analyte, the kit comprising a fusion protein comprising a structure of
- (An-B-Ax-C-Am)y
- wherein
- A independently comprises a peptide or protein domain that binds an analyte of interest;
- B comprises an entity that can produce a detectable signal;
- C comprises an entity that, when A is not bound to the analyte of interest, binds to B and modulates the signal production by B;
- n and m are each independently 0 or at least 1;
- x is an integer of 0 or at least 1;
- v is an integer of at least 1;
- “-” represents a covalent bond or a linker comprising or consisting of one or more amino acids;
- provided that if x is 0, n, m or both are at least 1.
Dependencies are inferred from the claim text.