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US 2014/0178888 A1Published application
Methods and compositions for exosome isolation
AssigneeLife Technologies Corporation
InventorsAlexander VLASSOV, Mu LI, Emily ZERINGER, Richard CONRAD
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Abstract
Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.
Claims19
A method for the isolation of exosomes from a biological fluid sample comprising:
- a) adding a volume-excluding polymer to the biological fluid sample,
- b) incubating the biological fluid sample with the volume-excluding polymer;
- and
- c) isolating the precipitated exosomes from the biological fluid sample.
1 more element
18-31. (canceled)
Dependencies are inferred from the claim text.