Composition and kit for diagnosing breast cancer including polynucleotide within vesicle, and method of diagnosing breast cancer using the same

A method of diagnosing breast cancer, the method comprising:

• providing a biological sample comprising a vesicle obtained from a subject;
• measuring an amount of microRNA (miRNA) in the sample, the microRNA being at least one selected from the group consisting of hsa-miR-126, hsa-miR-23a, hsa-miR-24, hsa-miR-19b, hsa-miR-103, hsa-miR-142-3p, hsa-miR-144, hsa-miR-15a, hsa-miR-185, hsa-miR-93, and hsa-miR-30c;
• comparing the measured amount of the microRNA with a control;
• and
• providing a breast cancer diagnosis based upon the comparison of the amount of the microRNA from the sample with a control,
• wherein providing a breast cancer diagnosis comprises determining if the measured amount of the microRNA from the biological sample is greater or lesser than the measured amount of the microRNA from the control,
• wherein when the control is an amount of microRNA from a biological sample from a subject having no breast tumor or a benign breast tumor, an increased amount of microRNA from the biological sample relative to the control is indicative of breast cancer in the subject, and
• wherein when the control is an amount of microRNA from a biological sample from a subject having breast cancer, a decreased amount of microRNA from the biological sample relative to a control is indicative of non-breast cancer diagnosis.