Method of preparing a reaction mixture and related products

A solution set for a polymerase chain reaction (PCR) assay, the solution set comprising

• a first reagent solution comprising
• a first colorant having a first color distinguishable in the visible spectrum, and
• at least one substance selected from the group consisting of a polymerase, a primer, a plurality of dNTPs, a sample solution containing or capable of receiving a template to be amplified, a fluorescent qPCR dye, and a probe, and
• a second reagent solution comprising
• a second colorant having a second color distinguishable in the visible spectrum, the second color different from the first color, and
• at least one substance selected from the group consisting of a polymerase, a primer, a plurality of dNTPs, a sample solution for receiving a template to be amplified, a fluorescent qPCR dye, and a probe not in the first reagent solution,
• at least the first and second reagent solutions on mixing forming a mixed solution, the mixed solution having, due to the first and second colorants, a third color different from the first and second colors and distinguishable in the visible spectrum.