Selective detection of neisseria meningitidis
AssigneeCenters for Disease Control and Prevention
InventorJennifer Thomas
Life of this patent
Estimating the term…
Abstract
A process for detecting Neisseria meningitidis nucleic acid in a sample is provided including producing an amplification product by amplifying Neisseria meningitidis nucleotide sequence of the sodC gene or mRNA using a forward primer of SEQ ID NO: 1, and a reverse primer of SEQ ID NO: 2, and detecting the amplification product to detect Neisseria meningitidis in the sample. Also provided are reagents and methods for detecting and distinguishing Neisseria meningitidis from other infectious agents. A kit is provided for detecting and quantifying Neisseria meningitidis in a sample.
Claims17
A process of detecting Neisseria meningitidis in a sample comprising:
- producing an amplification product by amplifying a Neisseria meningitidis nucleotide sequence using a forward primer that hybridizes to a region within the sodC gene or sodC mRNA of Neisseria meningitidis, and a reverse primer that hybridizes to a region within the sodC gene or sodC mRNA of Neisseria meningitidis, under conditions suitable for a polymerase chain reaction;
- and
- detecting said amplification product to detect the Neisseria meningitidis in said sample.
The process of 1 wherein said step of detecting is by gel electrophoresis, Southern blotting, liquid chromatography, mass spectrometry, liquid chromatography/mass spectrometry, static fluorescence, dynamic fluorescence, high performance liquid chromatography, ultra-high performance liquid chromatography, enzyme-linked immunoadsorbent assay, real-time PCR, nucleotide sequencing, or combinations thereof.
A kit for detecting Neisseria meningitidis infection comprising:
- a forward primer with sequence SEQ ID NO:
- 1;
- a reverse primer with SEQ ID NO:
- 2;
- and
- a probe.
An isolated oligonucleotide comprising the sequence selected from the group consisting of SEQ ID NO:
- 1;
- SEQ ID NO:
- 2, and SEQ ID NO:
- 3.
16-27. (canceled)
Dependencies are inferred from the claim text.