Photoinduced redox current (pirc) detection for dna sequencing using integrated transducer array
OwnerXing Su, Handong Liinventors
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Abstract
Photoinduced redox current (PIRC) detection for DNA sequencing using integrated transducer arrays is described. For example, a method of determining DNA sequencing using photoinduced redox current (PIRC) includes providing a template having a primer region and a second region of one or more unknown bases. The template is coupled to a transducer array. The method also includes incorporating a tagged nucleotide at the second region of the template. The method also includes exposing the template to a light source. The method also includes electrically detecting, by the transducer, a state of the template. The method also includes removing a photosensitizer tag of the tagged nucleotide.
Claims17
A method of determining DNA sequencing using photoinduced redox current (PIRC), the method comprising:
- providing a template having a primer region and a second region of one or more unknown bases, the template coupled to a transducer array;
- incorporating a tagged nucleotide at the second region of the template;
- exposing the template to a light source;
- electrically detecting, by the transducer, a state of the template;
- and
- removing a photosensitizer tag of the tagged nucleotide.
A method of determining DNA sequencing using photoinduced redox current (PIRC), the method comprising:
- providing an integrated transducer array having a plurality of immobilized DNA molecules thereon;
- delivering a reagent mixture comprising a plurality of photo-sensitizer tagged nucleotides to the integrated transducer array, in contact with the plurality of immobilized DNA molecules;
- exposing the integrated transducer array to a light source;
- and
- electrically detecting a state of the immobilized DNA molecules with the integrated transducer array.
-23. (canceled)
Dependencies are inferred from the claim text.