Integrated Assay that Combines Flow-Cytometry and Multiplexed HPV Genotype Identification
OwnerAndrew BIEBERICHinventor
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Abstract
A two part assay is disclosed that enables collection of both protein biomarker phenotype and specific HPV genotype data from within a clinically derived population of cervical epithelial cells. Presence of multiple transformation-associated protein biomarkers acts as a gating criterion for cell sorting, followed by application of a PCR protocol sensitive enough to detect and identify individual HPV types from within the cells captured during sorting. The workflow has been optimized to work with cells conventionally fixed in PreservCyt (Cytyc), and it can be performed on residual cells remaining in a stored sample after a Pap test has been performed.
Claims12
1-14. (canceled)
A method for detecting high-risk HPV genotypes in a cervical cell sample, the method comprising:
- collecting the sample from a cervical lavage or a Pap test;
- applying a polymerase chain reaction (PCR) master mix to the cells, the master mix comprising a plurality of PCR primers, the PCR primers comprising oligonucleotide sequences selected from the group consisting of:
- Sequence 1 TGGACCGGTCGATGTATGTCTTGT,
- Sequence 2 TACGCACAACCGAAGCGTAGAGTC,
- Sequence 3 AGTGTGACTCTACGCTTCGGTTGT,
- Sequence 4 GTGTGCCCATTAACAGGTCTTCCA,
- Sequence 5 ACTATAGAGGCCAGTGCCATTCGT,
- Sequence 6 TCGTCGGGCTGGTAAATGTTGATG,
- Sequence 7 CATCAACATTTACCAGCCCGACGA,
- Sequence 8 AAACAGCTGCTGGAATGCTCGAAG,
- Sequence 9 AACATAGGAGGAAGGTGGACAGGA,
- Sequence 10 GTGTGCTCTGTACACACAAACGAAG,
- Sequence 11 GAGGACACAAGCCAACGTTAAAGG,
- Sequence 12 GGTTCGTAGGTCACTTGCTGTACT,
- Sequence 13 GACAGTACCGAGGGCAGTGTAATA,
- Sequence 14 TACTTGTGTTTCCCTACGTCTGCGA,
- Sequence 15 GCGTGTGTATTATGTGCCTACGCT,
- Sequence 16 TTACACTTGGGTCACAGGTCGG, and the reverse complements of Sequences 1-16;
- executing a single multiplexed PCR amplification to produce a plurality of amplified, end-labeled amplicons;
- and
- identifying HPV types in the plurality of amplified, end-labeled amplicons using electrophoresis.
Dependencies are inferred from the claim text.