Potatoes with reduced cold-induced sweetening
AssigneeCellectis
InventorsLuc Mathis, Daniel F. Voytas, Feng Zhang, Benjamin Clasen, William Haun, Thomas Stoddard
Life of this patent
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Abstract
Materials and methods are provided for making plants (e.g., Solanum varieties) with decreased accumulation of reducing sugars and acrylamide in cold-stored potatoes, specifically, by making TALE-nuclease-induced mutations in genes encoding vacuolar invertase.
Claims35
A Solanum plant, plant part, or plant cell comprising a mutation in at least two VInv alleles endogenous to said plant, plant part, or plant cell, such that said plant, plant part, or plant cell has reduced expression of vacuolar invertase as compared to a control Solanum plant, plant part, or plant cell that lacks said mutation.
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9-12. (canceled)
A method for making a Solanum plant that has reduced cold-induced sweetening, wherein said method comprises:
- (a) contacting a population of Solanum plant cells comprising a functional VInv allele with a rare-cutting endonuclease targeted to an endogenous VInv sequence,
- (b) selecting, from said population, a cell in which at least two VInv alleles have been inactivated, and
- (c) growing said selected plant cell into a Solanum plant, wherein said Solanum plant has reduced cold-induced sweetening as compared to a control Solanum plant in which said VInv alleles have not been inactivated.
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A method for producing a food product, comprising:
- (a) providing a Solanum plant or plant part that (i) comprises a mutation in at least two VInv alleles endogenous to said plant or plant part, such that said plant, plant part, or plant cell has reduced expression of vacuolar invertase as compared to a control Solanum plant or plant part that lacks said mutation, and (ii) has been subjected to cold storage;
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- (b) producing a food product from said plant or plant part.
30-32. (canceled)
A food product produced from a Solanum plant or plant part that (i) comprises a mutation in each VInv allele endogenous to said plant or plant part, such that said plant, plant part, or plant cell has no functional VInv allele, and (ii) has been subjected to cold storage.
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40-41. (canceled)
Dependencies are inferred from the claim text.