Idna vaccines and methods for using the same
AssigneeMedigen, Inc.
InventorsPeter PUSHKO, Igor Lukashevich
Life of this patent
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Abstract
Described herein are iDNA vectors and vaccines and methods for using the same. The iDNA generates live attenuated vaccines in eukaryotic cells in vitro or in vivo for pathogenic RNA viruses, particularly yellow fever virus and Venezuelan equine encephalitis virus. When iDNA is injected into the vaccine recipient, RNA of live attenuated virus is generated by in vivo transcription in the recipient's tissues. This initiates production of progeny attenuated viruses in the tissues of the vaccine recipient, as well as elicitation of an effective immune response protecting against wild-type, non-attenuated virus.
Claims19
-44. (canceled)
A vector comprising:
- (a) DNA encoding an infectious RNA molecule;
- and
- (b) a eucaryotic RNA polymerase promoter;
- wherein the DNA encoding an infectious RNA molecule is operably linked to the RNA polymerase promoter.
A method of preparing a vaccine for immunizing a mammal against an infectious RNA virus, wherein the method comprises transfecting the vector of claim 1 into a eukaryotic cell and isolating clonally pure infectious viruses from a culture medium comprising the transfected eukaryotic cell, thereby obtaining a vaccine.
Dependencies are inferred from the claim text.