Multivalent antibody fragments and trimerized complexes thereof
AssigneeIndustrial Technology Research Institute
InventorsMin-Yuan CHOU, Chuan-Chuan HUANG, Hsiu-Chuan LI, Ya-Ping LAI
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Abstract
The present invention relates to a method for producing multivalent Fab fragments. In particular, the invention relates to a method for the generation of trimeric Fab fragments by co-expression of a gene construct comprising a heavy chain portion of a Fab fragment and an in-frame fused collagen-like peptide, and a gene construct consisting of a light chain portion of an IgG in mammalian cells. Uses of molecules generated using the method of the invention are also described.
Claims25
A method for producing a multivalent Fab fragment comprising the steps of co-expressing in a host cell:
- (1) a gene construct coding for an amino acid sequence comprising a heavy chain portion of a Fab fragment and an in-frame fused collagen-like peptide;
- and,
- (2) a gene construct coding for an amino acid sequence comprising a light chain variable region and the kappa light chain constant domain of human IgG.
A multivalent antibody fragment comprising:
- (1) an amino acid sequence comprising a heavy chain portion of a Fab fragment and an in-frame fused collagen-like peptide;
- and,
- (2) an amino acid sequence comprising a light chain variable region and the kappa light chain constant domain of human IgG.
A method for modulating biological activity of a ligand comprising:
- incubating a multivalent antibody fragment comprising:
- (1) an amino acid sequence comprising a heavy chain portion of a Fab fragment and an in-frame fused collagen-like peptide;
- and,
- (2) an amino acid sequence comprising a light chain variable region and the kappa light chain constant domain of human IgG
- with the ligand.
A method for treating autoimmune disease in a subject comprising:
- administering an effective amount of a multivalent antibody fragment comprising:
- (1) an amino acid sequence comprising a heavy chain portion of a Fab fragment and an in-frame fused collagen-like peptide;
- and,
- (2) an amino acid sequence comprising a light chain variable region and the kappa light chain constant domain of human IgG
- to a subject in need thereof.
A method for detecting a ligand comprising:
- incubating a trimeric multivalent antibody fragment complex comprising:
- (1) an amino acid sequence comprising a heavy chain portion of a Fab fragment and an in-frame fused collagen-like peptide;
- and,
- (2) an amino acid sequence comprising a light chain variable region and the kappa light chain constant domain of human IgG
- (3) a label,
- with the ligand;
- and detecting the binding of the trimeric multivalent antibody fragment complex.
Dependencies are inferred from the claim text.