Method for polyclonal immunoglobulin g production by human b cells
AssigneesHéma-Québec, Universite Laval
InventorsSonia Neron, Annie Roy, Jessie Farah Fecteau
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Abstract
This application relates to an in vitro method of producing a polyclonal IgG preparation. The method comprises (i) placing a polyclonal B-cell population enriched in IgG-secreting B cells in a culture medium; and (ii) culturing the polyclonal B-cell population under conditions enabling the production of the polyclonal IgG preparation from the polyclonal B-cell population. This improved method enables the production of antibodies (preferably IgG) and facilitates long-term culture of polyclonal B-cell populations.
Claims23
A human polyclonal IgG preparation prepared by an in vitro method, said method comprising:
- a) obtaining an initial human polyclonal B-cell population;
- b) prior to culture, substantially depleting IgM-secreting cells and IgD-secreting cells from the initial human polyclonal B-cell population so as to obtain a human polyclonal B-cell population enriched in IgG-secreting cells;
- c) placing the human polyclonal B-cell population enriched in IgG secreting cells in a culture medium;
- and
- d) culturing the human polyclonal B cell population enriched in IgG-secreting cells under conditions enabling the production of the human polyclonal IgG preparation from the polyclonal B cell population;
- wherein the human polyclonal B cell population of step b) has a concentration of IgM-secreting cells of less than 5% and a concentration of IgD-secreting cells of less than 5%.
A human polyclonal B-cell population prepared by an in vitro method, said method comprising:
- a) substantially depleting IgM-secreting cells and IgD-secreting cells from an initial human polyclonal B cell population prior to culture to provide a human polyclonal B-cell population enriched in IgG-secreting cells;
- b) placing the human polyclonal B-cell population enriched in IgG-secreting cells in a culture medium;
- and
- c) culturing the human polyclonal B-cell population enriched in IgG-secreting cells under conditions enabling the multiplication of the polyclonal B-cell population so as to obtain the human polyclonal B-cell population;
- wherein the human polyclonal B-cell population enriched in IgG-secreting cells of step b) has a concentration of IgM-secreting cells of less than 5% and a concentration of IgD-secreting cells of less than 5%.
Dependencies are inferred from the claim text.