Bacterial anti-phage defense systems
AssigneeYeda Research and Development Co. Ltd.
InventorsRotem SOREK, Hila Sberro, Azita Leavitt
Life of this patent
Estimating the term…
Abstract
An isolated polypeptide is disclosed comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 2773-5544 and 11089-11094, wherein the polypeptide has antimicrobial activity. Uses thereof for treating microbial infections are also disclosed.
Claims24
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 2773-5544 and 11089-11094, wherein the polypeptide has antimicrobial activity.
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 8317-11088, wherein the polypeptide protects a microbe from an activity of a toxin.
A method of killing a microbe, the method comprising contacting the microbe with an isolated peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 2773-5544, thereby killing the microbe.
An isolated bacterial population genetically modified to express a toxin and a cognate antitoxin thereof, the bacterial population being resistant to a lytic activity of a bacteriophage, wherein said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 2773-3117, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2773-2804, said antitoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:8317-8348, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2805-2871, said antitoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:8349-8415, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2872-2935, said antitoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:8416-8479, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2936-3030, said antitoxin comprises an amino acid selected from the group consisting of SEQ ID NO:8480-8574, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 3031-3078, said antitoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 8575-8622, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 3079-3087, said antitoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:8623-8631, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 3088-3094, said antitoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:8632-8638, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 3095-3109, said antitoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:8639-8653, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 3110-3117, said antitoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:8654-8661.
A method of protecting a bacterial population from phage attack, the method comprising expressing in the bacterial population a toxin and a cognate antitoxin thereof, wherein said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 2773-3117, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2773-2804, said antitoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:8317-8348, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2805-2871, said antitoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:8349-8415, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2872-2935, said antitoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:8416-8479, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2936-3030, said antitoxin comprises an amino acid selected from the group consisting of SEQ ID NO:8480-8574, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 3031-3078, said antitoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 8575-8622, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 3079-3087, said antitoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:8623-8631, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 3088-3094, said antitoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:8632-8638, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 3095-3109, said antitoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:8639-8653, wherein when said toxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 3110-3117, said antitoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NO:8654-8661.
A nucleic acid system comprising:
- (i) a first isolated polynucleotide which encodes a toxin, said toxin having an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 2773-5544;
- and
- (ii) a second isolated polynucleotide which encodes a cognate antitoxin to said toxin, said antitoxin having an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 8317-11088.
Dependencies are inferred from the claim text.