Cellular compositions for tissue engineering
AssigneeThe Regents of the University of California
InventorsJames A. Byrne, Jeffrey C. Wang
Life of this patent
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Abstract
Cell compositions for tissue engineering are provided which contain a population of autologous, minimally passaged dermal fibroblasts in combination with a tissue engineering matrix or scaffold, or material forming a matrix or scaffold. In one embodiment, the population of fibroblasts is genetically engineered to secrete a therapeutic protein in an amount effective to induce tissue growth or tissue repair when the cell composition is transplanted into a subject in need thereof. For example, the therapeutic protein can be a bone morphogenic protein when the tissue to be treated is bone tissue. A preferred bone morphogenic protein is BMP-2.
Claims19
A method of stimulating bone formation in the body, the method comprising:
- (a) genetically modifying autologus cells to express bone formation stimulating proteins;
- and
- (b) implanting said genetically modified autologus cells in a location in a body of a patient identified for bone formation to induce bone formation.
A method of bone growth stimulation in the body of a patient, the method comprising:
- (a) culturing a sample of cells from a patient;
- (b) genetically modifying the cultured cells to express bone growth stimulating proteins;
- (c) associating the genetically modified cells with an extracellular matrix;
- and
- (d) implanting the extracellular matrix and the cells in the body of the patient.
A method of spinal fusion in the body of a patient, the method comprising:
- (a) culturing a sample of fibroblast cells from a patient;
- (b) exposing the cultured fibroblast cells to a lentiviral vector with a BMP-2 gene to produce genetically modified cultured cells expressing BMP-2;
- (c) associating the genetically modified cells with an extracellular matrix;
- and
- (d) implanting the extracellular matrix and the cells between spinal vertebrae in the body of the patient.
Dependencies are inferred from the claim text.