Individualized bacterial treatment of pancreatic cancer
AssigneeAntiCancer, Inc.
InventorsMing Zhao, Fuminari Uehara
Life of this patent
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Abstract
An individualized bacterial treatment of cancer is provided. The treatment includes a strain of bacteria modified by in-vivo passage through tumor grafts in experimental animals, where the modified strain exhibits enhanced cancer cell-targeting of a specific malignancy arising in a unique individual to the corresponding parent strain of bacteria. The treatment uses this modified strain for the treatment human solid-tumor malignancies by inoculating an individual with a quantity of the strain; and repeating inoculations at periodic intervals where repeated inoculations tend to progressively eliminate the solid tumor malignancy in the individual.
Claims35
A strain of enhanced cancer cell-targeting bacteria for individualized cancer therapy comprising:
- A modified strain of bacteria, wherein said modified strain is created by at least one in-vivo passage through tumor grafts in experimental animals;
- and
- said modified strain exhibits enhanced cancer cell targeting of a specific malignancy arising in a unique individual compared to a corresponding unmodified strain of bacteria.
A method for creating a strain of enhanced cancer cell-targeting bacteria for individualized cancer therapy, said method comprising:
- (a) harvesting cancer cells from an individual;
- (b) transplanting a quantity of said cancer cells into a quantity of experimental animals;
- (c) incubating the experimental animals to establish a cancer tissue graft within the experimental animals;
- (d) inoculating a bacterial strainA1 into a said experimental animal containing established said cancer tissue graft;
- (e) removing said cancer tissue graft from said experimental animal inoculated with said bacterial strainA1 following a period of incubation of the inoculated said experimental animal;
- (f) extracting bacteriaR from said cancer tissue graft removed from the experimental animal inoculated with said bacterial strain;
- (g) incubating said bacteriaR under conditions suitable for bacterial multiplication;
- and
- (h) selecting a said bacteriaR.
A method for treatment of human malignancies using a strain of enhanced cancer cell-targeting bacteria for individualized cancer therapy, said method comprising:
- (a) harvesting cancer cells from an individual with a malignancy;
- (b) creating tumor xenografts in immunologically deficient experimental animals with harvested said cancer cells;
- (c) selecting a strain of enhanced cancer cell-targeting bacteria by at least one in-vivo passage of said bacteria through said tumor xenografts within said experimental animals;
- (d) performing extraction, isolation, and propagation of said strain;
- (e) performing inoculation of said individual with a quantity of said strain;
- and
- (f) repeating inoculations at periodic intervals of a quantity of said strain in said individual wherein repeated inoculations tend to progressively eliminate said solid tumor malignancy in said individual.
Dependencies are inferred from the claim text.