Polypeptides
AssigneeAffibody AB
InventorsFredrik Nilsson, Tove Eriksson, Andreas Jonsson, Stefan Stahl, Mikaela Friedman
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Abstract
This invention relates to polypeptides which bind to EGFR family receptors and to applications of those polypeptides in medicine, veterinary medicine, diagnosis, diagnostics, and imaging.
Claims36
-79. (canceled)
A diagnostic method for determining the presence of an epidermal growth factor receptor (EGFR) in a mammalian subject, the method including contacting the subject, or a sample derived from the subject, with an EGFR-binding polypeptide, alone or in combination with a detectable agent, said polypeptide comprising an epidermal growth factor receptor binding motif, EBM, which motif consists of an amino acid sequence selected from:
- i) EX2X3X4AX6X7EIR X11LPNLNGWQX20 TAFIX25SLX28D,
- wherein, independently of each other,
- X2 is selected from M, V, L and I;
- X3 is selected from W, D and E;
- X4 is selected from I, V, G, S, M, L, A, T, N and D;
- X6 is selected from W, V and I;
- X7 is selected from D, E, N and K;
- X11 is selected from D, N, E, Y and S;
- X20 is selected from M, L and F;
- X25 is selected from A, S and G;
- and
- X28 is selected from L, V and F;
- and
- ii) an amino acid sequence which has at least 85% identity to the sequence defined in i);
- the EGFR-binding polypeptide binding to EGFR such that the KD value of the interaction is at most 10 μM.
Dependencies are inferred from the claim text.