Method for the detection of incorrect deposition on a maldi sample support
AssigneeBruker Daltonik GmbH
InventorMarcus GRIEGER
Life of this patent
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Abstract
The invention relates to a method for the detection of incorrect deposition on a MALDI sample support with several separate sample sites, where after the preparation on the sample support, an area located between two sample sites is sampled with a desorption laser, and a signal of an ion detector in a mass spectrometer is acquired in temporal relation to the sampling. The signal is examined for the presence of a signal which indicates incorrect deposition. An advantage of the method is particularly that it can be carried out using a MALDI ion source and a connected mass analyzer, and that it requires little procedural effort.
Claims12
A method for the detection of an incorrect deposition on a MALDI sample support which has several separate sample sites, wherein during or after the preparation on the sample support, an area located between two sample sites is sampled with a desorption laser, and a signal of an ion detector in a mass spectrometer is acquired in temporal relation to the sampling, and the signal is examined for the presence of a signal shape which indicates incorrect deposition.
A method for the mass-spectrometric detection of analyte molecules ionized with the aid of MALDI, comprising:
- providing a sample support having several separate sample sites and being prepared with samples;
- directing a desorption laser at different sample sites in succession and activating it in order to ionize and detect any analyte molecule(s) prepared there;
- and
- sampling an area around a next sample site to be bombarded with the desorption laser in order to deduce the presence of contamination from a signal of an ion detector in a mass analyzer, which is interrogated in temporal relation to the sampling.
Dependencies are inferred from the claim text.