Method of a test strip detecting concentration of an analyte of a sample, three-electrode test strip, and method of utilizing a test strip detecting diffusion factor

A test strip with three electrodes, the test strip comprising:

• a substrate;
• and
• a reaction region formed on a first end of the substrate, and an enzyme is coated in the reaction region, wherein when a sample is placed in the reaction region, an analyte reacts with the enzyme to generate a plurality of electrons, and the plurality of electrons are transferred through a mediator, the reaction region comprising:
• a working electrode for receiving an electrical signal when the sample is placed in the reaction region, generating a first current according to the electrical signal during a first period, and generating a second current according to the electrical signal during a third period behind a second period, wherein a second polarity of the electrical signal during the second period is inverse to a first polarity of the electrical signal during the first period and a third polarity of the electrical signal during the third period, wherein the mediator generates an intermediate according to the electrical signal during the second period;
• a reference electrode for receiving a reference voltage when the sample is placed in the reaction region;
• and
• a counter electrode for receiving a floating voltage when the sample is placed in the reaction region to satisfy a current generated by the working electrode during the first period, the second period, and the third period, wherein the current comprises the first current and the second current;
• wherein the first current is used for calculating initial concentration of the analyte, the second current is used for calculating a diffusion factor of the intermediate, and the diffusion factor is used for correcting the initial concentration to generate new concentration of the analyte.

A method of a test strip detecting concentration of an analyte of a sample, wherein the test strip comprises a substrate and a reaction region, the reaction region comprises a working electrode, a reference electrode, and a counter electrode, and an enzyme is coated in the reaction region, the method comprising:

• placing the sample in the reaction region, wherein the analyte reacts with the enzyme to generate a plurality of electrons, and the plurality of electrons are transferred to the working electrode through a mediator;
• applying an electrical signal to the working electrode;
• measuring a first current through the working electrode during a first period;
• the mediator generating an intermediate according to the electrical signal during a second period;
• measuring a second current through the working electrode during a third period, wherein the electrical signal has a second polarity and a non-polarity during the second period, and the second polarity is inverse to a first polarity of the electrical signal during the first period and a third polarity of the electrical signal during the third period;
• calculating initial concentration of the analyte according to the first current;
• calculating a diffusion factor of the intermediate in the sample according to the second current;
• and
• correcting the initial concentration to generate new concentration of the analyte according to the diffusion factor.

A method of utilizing a test strip to detect a diffusion factor of a mediator in a sample, wherein the test strip comprises a reaction region, and the reaction region comprises a working electrode, a reference electrode, and a counter electrode, the method comprising:

• placing the sample in the reaction region;
• applying an electrical signal to the working electrode;
• the mediator generating an intermediate according to the electrical signal during a first period;
• measuring a first current through the working electrode during a second period behind the first period, wherein a second polarity of the electrical signal during the second period is inverse to a first polarity of the electrical signal during the first period;
• and
• calculating the diffusion factor of the intermediate in the sample according to the first current.

A method of utilizing a test strip to detect a diffusion factor of a mediator in a sample, wherein the test strip comprises a reaction region, and the reaction region comprises a working electrode, a reference electrode, and a counter electrode, the method comprising:

• placing the sample in the reaction region;
• applying an electrical signal to the working electrode;
• the mediator generating an intermediate according to the electrical signal during a first period;
• measuring a first current through the working electrode during a second period behind the first period, wherein the electrical signal has a first polarity and a non-polarity during the first period, and the first polarity is inverse to a second polarity of the electrical signal during the second period;
• and
• calculating the diffusion factor of the intermediate in the sample according to the first current.