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US 2014/0174931 A1Published application
Nucleic acid sample preparation
AssigneeBiological Dynamics, Inc.
InventorsRajaram KRISHNAN, David CHARLOT, Eugene TU, James MCCANNA, Lucas KUMOSA, Paul SWANSON, Robert TURNER, Kai YANG, Irina DOBROVOLSKAYA
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Abstract
The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.
Claims21
A method for isolating a nucleic acid from a fluid, the method comprising:
- a. applying the fluid to a device, the device comprising an array of electrodes capable of establishing an AC electrokinetic field region;
- b. concentrating cells and/or other particulate material in the fluid in a first AC electrokinetic field region, wherein the first AC electrokinetic field region is a first dielectrophoretic high field region and the conductivity of the fluid is less than 500 mS/m;
- c. isolating nucleic acid in a second AC electrokinetic field region, wherein the second AC electrokinetic field is a second dielectrophoretic high field region;
- and
- d. flushing the concentrated cells and/or other particulate material from the first AC electrokinetic field region.
2 more elements
A method for isolating a nucleic acid from a fluid, the method comprising:
- a. applying the fluid to a device, the device comprising an array of electrodes capable of establishing an AC electrokinetic field region;
- b. concentrating cells and/or other particulate material in the fluid in a first AC electrokinetic field region, wherein the first AC eletrokinetic field region is a first dielectrophoretic high field region and the conductivity of the fluid is less than 500 mS/m;
- c. isolating nucleic acid in a second AC electrokinetic field region, wherein the second AC electrokinetic field is a second dielectrophoretic high field region;
- d. flushing the concentrated cells and/or other particulate material from the first AC electrokinetic field region;
- e. degrading residual proteins and/or material;
- and
- f. flushing the degraded residual proteins and/or material from the isolated nucleic acid.
4 more elements
Dependencies are inferred from the claim text.