US 2014/0173783 A1 · PRECISION GENE TARGETING TO A PARTICULAR LOCUS IN MAIZE

Abstract

The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

Claims20

Dependent claims

A method for integrating one or more exogenous nucleic acid sequences into the genome of a maize cell having an E32 locus, said method comprising:

making a double-stranded cleavage in the E32 locus using a site specific nuclease, ligating into the cleavage a polynucleotide comprising the one or more exogenous sequences into the genome of the maize cell within the E32 locus.

Dependencies are inferred from the claim text.

Facts

Application

14/105,563Filed 13 Dec 2013

Priority

13 Dec 2012

Published

19 Jun 2014

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12N 15/8213
Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for…:…
C12N 9/1241
Transferring phosphorus containing groups, e.g. kinases: nucleotidyltransferases
C12Y 207/07047
Nucleotidyltransferases: streptomycin 3''-adenylyltransferase
C12N
Microorganisms or enzymes

Precision gene targeting to a particular locus in maize

Life of this patent

Precision gene targeting to a particular locus in maize

Life of this patent

Abstract

Claims20