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US 2014/0162363 A1Published application
Method of cell cultures and device for implementing it
AssigneeArtelis S.A.
InventorJose Antonio CASTILLO FERNANDEZ
Life of this patent
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Abstract
The invention generally provides a cell culture vessel having at least one first zone and at least one second zone, wherein the first zone is a transfer zone for a culture medium which essentially contains no cells and the second zone is a cell culture zone. The invention further includes methods utilizing the cell culture vessel.
Claims18
A cell culture device comprising:
- a culture vessel provided with a cover, wherein there is situated at least one first zone and at least one second zone, wherein said first zone is a transfer zone for the culture medium essentially containing no cells and said second zone is a cell culture zone, means of circulating the culture medium, allowing circulation of the culture medium through the culture zone, said culture zone comprising a bottom wall and a top wall, wherein each wall is provided with orifices allowing a transfer of culture medium essentially free from cells, characterised in that:
- it also comprises at least one third and at least one fourth zone, wherein both are culture medium transfer zones essentially free from cells, wherein said third zone is in medium communication with the first and second zone and wherein said fourth zone is in medium communication with the second zone and with the first zone via the culture medium circulation means, and in that the culture medium circulation means allow a circulation of the culture medium from bottom to top in said second culture zone.
A method of culturing cells in a culture vessel with culture medium circulation, comprising the steps of:
- introduction of culture medium (M) into culture medium circulation means, discharge of culture medium (M) from said culture medium circulation means, at least one first transfer of culture medium (M) into a first culture medium upward transfer zone, at least one second transfer of culture medium (M) into a second cell culture zone, characterised in that it also comprises:
- at least one third culture medium transfer subsequent to said first culture medium transfer into a third culture medium transfer zone, by overflowing from the first culture medium transfer zone to the third culture medium transfer zone, at least one fourth culture medium transfer subsequent to said second culture medium transfer into a fourth culture medium transfer zone, by overflowing from the second culture zone into the fourth culture medium transfer zone, and in that said second culture medium transfer is a downward culture medium transfer.
Dependencies are inferred from the claim text.