Methods for generation of antibodies
AssigneeNational Jewish Health
InventorsYosef Refaeli, Brian C. Turner
Life of this patent
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Abstract
This invention generally relates to methods for the production of antibody producing cells and antibodies in protooncogene expressing animals. The invention also relates to methods for the efficient production of antibodies specific for antigens that are normally subject to immunological constraints such as self tolerance. The invention further relates to the production of antibody producing cells and antibodies without the need for the conventional fusing of antibody producing B cells with a myeloma fusion partner.
Claims21
1-61. (canceled)
A method for preparing an antibody producing cell with antibody specificity for an antigen of interest, comprising:
- exposing the antigen of interest to a non-human animal that overexpresses MYC in an inducible manner, wherein the overexpression of MYC is regulated in a temporal manner, such that MYC is not overexpressed until the production of antibodies is desired, and wherein overexpression of MYC enables B cells to produce antibodies specific for the antigen of interest.
An antibody producing cell with antibody specificity for an antigen of interest, comprising:
- a B cell that overexpresses MYC in an inducible manner, wherein the B cell is engineered to express the antigen of interest, and wherein temporal overexpression of MYC enables the production of antibodies specific for the antigen of interest.
A hematopoietic stem cell capable of giving rise to a population of B cells having antibodies specific for an antigen of interest, comprising:
- an inducible MYC that can be temporally overexpressed;
- and a transduced antigen of interest;
- wherein overexpression of MYC in the population of B cells enables the B cells to produce antibodies specific for the antigen of interest.
Dependencies are inferred from the claim text.