# US 2014/0157458 A1 · Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0157458 A1 - Document ID: US20140157458A1 - Status: Published application - Kind: A1 - Application number: 14/089,260 - Filed: 2013-11-25 - Published: 2014-06-05 - Earliest claimed: 2009-10-22 - Assignees: Sangamo BioSciences, Inc.; Dow AgroSciences LLC - Applicants: Sangamo BioSciences, Inc.; Dow AgroSciences LLC - Inventors: Russell DeKelver; Manju Gupta; Jeffrey C. Miller; Stephen Novak; Joseph F. Petolino - Web page: https://patentgrep.com/patent/US20140157458A1 ## CPC - C12N 15/8218: {Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]} - C07K 14/415: from plants - C12N 9/22: Ribonucleases {[RNase]; Deoxyribonucleases [DNase]} - C12N 15/8216: {Methods for controlling, regulating or enhancing expression of transgenes in plant cells} - C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition} - C12Y 203/01041: Beta-ketoacyl-acyl-carrier-protein synthase I (2.3.1.41) - C12Y 301/00: Hydrolases acting on ester bonds (3.1) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - C12Y: ENZYMES ## Abstract The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided. ## Claims ### Claim 1 A non-naturally occurring zinc finger protein that modulates expression of at least one endogenous Fatty acid thioesterase (Fat) plant gene. ### Claim 2 (depends on 1) A fusion protein comprising a zinc finger protein of claim 1 and a functional domain. ### Claim 3 (depends on 2) The fusion protein of claim 2, wherein the functional domain is a transcriptional regulatory domain or a cleavage domain. ### Claim 4 (depends on 1) The zinc finger protein or fusion protein of claim 1, wherein the plant gene is contained within a Brassica plant. ### Claim 5 (depends on 4) The zinc finger protein of claim 4, wherein the zinc finger protein modulates expression of a FatA gene. ### Claim 6 (depends on 4) The zinc finger protein of claim 4, wherein the zinc finger protein modulates expression of a FatB gene. ### Claim 7 (depends on 1) A polynucleotide encoding one or more zinc finger proteins of claim 1. ### Claim 8 (depends on 7) A method of modifying one or more genes involved in fatty acid biosynthesis in a plant cell, the method comprising: introducing, into the plant cell, one or more expression vectors comprising at least one polynucleotide according to claim 7, under conditions such that the one or more zinc finger proteins are expressed and the one or more Fat genes are modified. ### Claim 9 (depends on 8) The method of claim 8, wherein the modification comprises modulating expression of at least one Fat gene. ### Claim 10 (depends on 9) The method of claim 9, wherein expression of at least one Fat gene is activated. ### Claim 11 (depends on 9) The method of claim 9, wherein expression of at least one Fat gene is repressed. ### Claim 12 (depends on 8) The method of claim 8, wherein the polynucleotide encodes a zinc finger nuclease and at least one Fat gene is cleaved. ### Claim 13 (depends on 12) The method of claim 12, further comprising the step of introducing a donor nucleic acid such the donor vector is introduced into the site of cleavage by homologous recombination. ### Claim 14 (depends on 8) A plant cell comprising at least one Fat gene that has been modified by any of the methods of claim 8. ### Claim 15 (depends on 14) The plant cell of claim 14, wherein fatty acid content within a seed and fatty acid content in the seed is modified. ### Claim 16 (depends on 14) A plant comprising at least one cell according to claim 14. ### Claim 17 (depends on 16) The seed or progeny of a plant according to claim 16. ### Claim 18 (depends on 1) A plant comprising at least one cell comprising the zinc finger protein of claim 1. ### Claim 19 (depends on 7) A plant comprising at least one cell comprising the polynucleotide of claim 7. ### Claim 20 (depends on 18) The seed or progeny of a plant according to claim 18.