Pest resistant plants
AssigneeKeygene N.V.
InventorsRobert Cornelis Schuurink, Micheal Abertus Haring, Petronella Martina Bleeker
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Abstract
The disclosure provides an isolated nucleic acid molecule encoding a 7-epizingiberene synthase, a chimeric gene comprising said nucleic acid molecule, vectors comprising the same, as well as isolated 7-epizingiberene synthase proteins themselves. In addition, transgenic plants and plant cells comprising a gene encoding a 7-epizingiberene synthase, optionally integrated in its genome, and methods for making such plants and cells, are provided. Especially Solanaceae plants and plant parts (seeds, fruit, leaves, etc.) with enhanced insect pest resistance are provided.
Claims22
An isolated protein comprising the amino acid sequence of SEQ ID NO:
- 1 or an amino acid sequence comprising at least 92% amino acid sequence identity to the amino acid sequence of SEQ ID NO:1 over the entire length.
A recombinant nucleic acid sequence comprising a heterologous promoter operably linked to a nucleic acid sequence selected from the group consisting of:
- a) a nucleic acid sequence of SEQ ID NO:
- 2;
- b) a nucleic acid sequence that encodes a polypeptide comprising an amino acid sequence of SEQ ID NO:
- 1;
- c) a nucleic acid sequence that is at least 96% identical to the nucleic acid sequences of (a) or (b), and encodes a 7-epizingiberene synthase;
- d) a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:1 wherein at least one amino acid is substituted, deleted, inserted or added and wherein the polypeptide is functionally equivalent to the polypeptide consisting of the amino acid sequence of SEQ ID NO:
- 1 and
- e) a nucleic acid sequence that hybridizes under stringent conditions to the nucleic acid sequences of (a), (b), or (c), and encodes a 7-epizingiberene synthase.
(canceled)
A method for producing 7-epizingiberene from zFPP in a host cell, comprising:
- a) introducing into said host cell a first nucleic acid sequence encoding a zFPS as shown in SEQ ID NO:6 or an amino acid sequence comprising at least 80% amino acid sequence identity to the amino acid sequence of SEQ ID NO:6 over the entire length, and a second nucleic acid sequence encoding a 7-epizingiberene synthase comprising the amino acid sequence of SEQ ID NO:
- 1 or an amino acid sequence comprising at least 92% amino acid sequence identity to the amino acid sequence of SEQ ID NO:1;
- b) culturing the transformed cell in suitable conditions for the expression of said first and said second nucleic acid sequences;
- and,
- c) optionally, collecting the zFPP and/or the 7-epizingiberene contained in said cell and/or in the culture medium.
A transgenic plant, plant cell, seed or fruit, comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:1 or an amino acid sequence comprising at least 92% amino acid sequence identity to the amino acid sequence of SEQ ID NO:1 over the entire length.
A Solanum lycopersicum plant, plant cell, seed or fruit, comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:1 or an amino acid sequence comprising at least 92% amino acid sequence identity to the amino acid sequence of SEQ ID NO:1 over the entire length.
A method for producing a transgenic plant having enhanced insect pest resistance compared to a non-transgenic control plant, said method comprising the steps of:
- (a) transforming a plant or plant cell with a nucleic acid molecule encoding the amino acid sequence of SEQ ID NO:
- 1 or an amino acid sequence comprising at least 92% amino acid sequence identity to the amino acid sequence of SEQ ID NO:1 over the entire length, operably linked to a promoter active in plant cells, and
- (b) regenerating a plant.
(canceled)
A method for identifying a genomic polymorphism between Solanum habrochaites and species of the Solanum type that are sexually compatible with Solanum habrochaites comprising detecting a genomic polymorphism with molecular markers comprising all or part of the gene encoding the amino acid sequence of SEQ ID NO:1 or an amino acid sequence having at least 92% sequence identity with the amino acid sequence of SEQ ID NO:1 so as to control the introgression of the corresponding gene in said species.
An isolated, synthetic or recombinant nucleic acid sequence comprising a nucleic acid sequence selected from the group consisting of:
- a) a nucleic acid sequence of SEQ ID NO:
- 2;
- b) a nucleic acid sequence that is at least 96% identical to the nucleic acid sequence of (a) and encodes 7-epizingiberene synthase;
- and
- c) a nucleic acid sequence that hybridizes under stringent conditions to the nucleic acid sequences of (a) and (b) and encodes a z-epizingiberene synthase.
Dependencies are inferred from the claim text.