# US 2014/0157454 A1 · Powdery Mildew Resistance Providing Genes in Cucumis Sativus > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0157454 A1 - Document ID: US20140157454A1 - Status: Published application - Kind: A1 - Application number: 14/002,284 - Filed: 2012-02-20 - Published: 2014-06-05 - Earliest claimed: 2011-03-01 - Assignees: Keygene N.V.; Enza Zaden Beheer B.V. - Inventors: Paul Johan Diergaarde; Leonora Johanna Gertruda Van Enckevort; Karin Ingeborg Posthuma; Marinus Willem Prins - Web page: https://patentgrep.com/patent/US20140157454A1 ## CPC - C12N 15/8282: {for fungal resistance} - C07K 14/415: from plants - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract The present invention relates to powdery mildew resistance providing genes of the Cucumis family, and especially Cucumis sativus, wherein said resistance is provided by impairment of the present genes. Further, the present invention relates plants comprising the present impaired resistance conferring genes and seeds, embryos or other propagation material thereof. Especially, the present invention relates to powdery mildew resistance conferring genes, wherein the amino acid sequence encoded by said resistance conferring gene is selected from the group consisting of SEQ ID No. 2, SEQ ID No. 4, SEQ ID No. 6, SEQ ID No. 8, SEQ ID No. 10, SEQ ID No. 12, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 18, SEQ ID No. 20 and SEQ ID No. 22, and amino acid sequences with more than 70% identity, preferably more than 80% identity, more preferably more than 90% identity, and most preferably more than 95% identity. ## Claims ### Claim at position 1 1-11. (canceled) ### Claim 12 An isolated cucumber plant that is resistant to powdery mildew comprising in its genome an impaired powdery-mildew susceptibility gene, wherein the presence of the impaired powdery-mildew susceptibility gene is determinable by a decrease, absence, or loss of function of at least one of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, and SEQ ID NO: 22. ### Claim 13 (depends on 12) The isolated cucumber plant according to claim 12, wherein the impairment is one or more mutations, and wherein the one or more mutations cause the absence of a protein. ### Claim 14 (depends on 12) The isolated cucumber plant according to claim 12, wherein the impairment is one or more mutations, and wherein the one or more mutations cause a non-functioning protein. ### Claim 15 (depends on 12) The isolated cucumber plant according to claim 12, wherein the impairment is gene silencing. ### Claim 16 (depends on 12) Seeds, fruits, plant parts, or propagation material of the cucumber plant according to claim 12. ### Claim 17 A cDNA transcribed from a nucleotide sequence selected from SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, and SEQ ID NO: 21. ### Claim 18 A method for obtaining a cucumber plant that is resistant to powdery mildew, comprising introducing an impairment to at least one nucleotide sequence selected from SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, and SEQ ID NO: 21 in a cucumber plant that is susceptible to powdery mildew. ### Claim 19 (depends on 18) The method according to claim 18, wherein the impairment is one or more mutations, and wherein the one or more mutations cause the absence of a protein. ### Claim 20 (depends on 18) The method according to claim 18, wherein the impairment is one or more mutations, and wherein the one or more mutations cause a non-functioning protein. ### Claim 21 (depends on 18) The method according to claim 18, wherein the impairment is gene silencing. ### Claim 22 (depends on 18) The method according to claim 18, wherein the presence of the impairment is determinable by a decrease, absence, or loss of function of at least one amino acid sequence selected from SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, and SEQ ID NO: 22. ### Claim 23 (depends on 22) The method according to claim 22, wherein, when the nucleotide sequence is SEQ ID NO: 1, the amino acid sequence is SEQ ID NO: 2, when the nucleotide sequence is SEQ ID NO: 3, the amino acid sequence is SEQ ID NO: 4, when the nucleotide sequence is SEQ ID NO: 5, the amino acid sequence is SEQ ID NO: 6, when the nucleotide sequence is SEQ ID NO: 7, the amino acid sequence is SEQ ID NO: 8, when the nucleotide sequence is SEQ ID NO: 9, the amino acid sequence is SEQ ID NO: 10, when the nucleotide sequence is SEQ ID NO: 11, the amino acid sequence is SEQ ID NO: 12, when the nucleotide sequence is SEQ ID NO: 13, the amino acid sequence is SEQ ID NO: 14, when the nucleotide sequence is SEQ ID NO: 15, the amino acid sequence is SEQ ID NO: 16, when the nucleotide sequence is SEQ ID NO: 17, the amino acid sequence is SEQ ID NO: 18, when the nucleotide sequence is SEQ ID NO: 19, the amino acid sequence is SEQ ID NO: 20, and when the nucleotide sequence is SEQ ID NO: 21, the amino acid sequence is SEQ ID NO: 22.