# US 2014/0155871 A1 · Method for preparing corneal donor tissue for refractive eye surgery utilizing the femtosecond laser

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- Number: US 2014/0155871 A1
- Document ID: US20140155871A1
- Status: Published application
- Kind: A1
- Application number: 13/891,088
- Filed: 2013-05-09
- Published: 2014-06-05
- Earliest claimed: 2012-05-10
- Assignees: James Stuart Cumming
- Inventors: James Stuart Cumming
- Web page: https://patentgrep.com/patent/US20140155871A1

## CPC

- A61F 9/00834: {Inlays; Onlays; Intraocular lenses [IOL]}
- A61F 2/142: {Cornea, e.g. artificial corneae, keratoprostheses or corneal implants for repair of defective corneal tissue}
- A61F 2/1451: {Inlays or onlays}
- A61F: FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS (dental prosthetics A61C)

## Abstract

A method of accurately fabricating a non-human donor corneal tissue for implantation into a recipient human cornea, the method comprising the steps of: removing corneal tissue from a donor with a femtosecond laser; placing the corneal tissue in a fixative solution for a selected time interval to cross-link the collagen fibrils in the tissue and prevent swelling of the corneal tissue; and shaping the tissue to provide a conical inlay of a selected shape and thickness having one or more radial extensions. The method is such that the corneal inlay may be attached to the peripheral corneal and/or the sclera. The method is such that the corneal inlay may be stored for a period of up to two years prior to attachment.

## Claims

### Claim 1

A method of accurately fabricating a non-human donor conical tissue for implantation into a recipient human cornea, the method comprising the steps of:
removing cortical tissue from a donor with a femtosecond laser;
placing the corneal tissue in a fixative solution for a selected time interval to cross-link the collagen fibrils in the tissue and prevent swelling of the corneal tissue; and
shaping the tissue to provide a conical inlay of a selected shape and thickness having one or more radial extensions.

### Claim 2 (depends on 1)

The method of claim 1, wherein the fixative solution comprises gluteraldehyde.

### Claim 3 (depends on 1)

The method of claim 1, wherein the fixative solution comprises 0.1 M phosphate buffer.

### Claim 4 (depends on 1)

The method of claim 1, wherein the fixative solution comprises 0.5% to 4.0% gluteraldehyde in 0.1 M phosphate buffer.

### Claim 5 (depends on 1)

The method of claim 1, wherein the selected time interval in which the tissue is placed in the fixative solution is up to two weeks.

### Claim 6 (depends on 1)

The method of claim 1, further comprising the intermediate step of:
washing the corneal tissue in a physiological solution prior to shaping,

### Claim 7 (depends on 1)

The method of claim 1, further comprising the intermediate step of:
attaching sutures to the conical inlay after shaping so as to reduce astigmatism when the conical inlay is attached to a human sclera.

### Claim 8 (depends on 7)

The method of claim 7, wherein the sutures are attached to the radial extensions.

### Claim 9 (depends on 1)

The method of claim 1, wherein the corneal tissue is shaped while in a non-frozen state.

### Claim 10 (depends on 1)

The method of claim 1, wherein the corneal tissue is shaped using the femtosecond laser.

### Claim 11 (depends on 1)

The method of claim 1, wherein the corneal tissue is that of an animal selected from the group of: pigs, cows, rabbits, cats, dogs, primates, cetacean dolphins, sharks, mammals and chondrychthes,

### Claim 12 (depends on 1)

The method of claim 1, wherein the corneal tissue may be in the form of a partial or full thickness graft.

### Claim 13 (depends on 1)

The method of claim 1, wherein the extensions may be inserted into the sclera of the recipient cornea so as to support an ectastic posterior cortical layer of the recipient cornea.

### Claim 14 (depends on 13)

The method of claim 13, wherein the extensions may be sutured to the sclera.

### Claim 15 (depends on 1)

The method of claim 1, wherein the shaped conical inlay is of a suitable thickness to permit reshaping with a femtosecond laser after it has been attached to the human cornea so as to adjust the power of the corneal inlay.

### Claim 16 (depends on 1)

The method of claim 1, wherein the step of placing the corneal tissue in a fixative solution for a selected time interval causes the corneal inlay to be able to be stored for a period of up to two years.