# US 2014/0155460 A1 · Micro-rna's that regulate muscle cells

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- Number: US 2014/0155460 A1
- Document ID: US20140155460A1
- Status: Published application
- Kind: A1
- Application number: 14/088,956
- Filed: 2013-11-25
- Published: 2014-06-05
- Earliest claimed: 2005-04-04
- Assignees: Board of Regents, The University of Texas System
- Applicants: The Board of Regents of the University of Texas System
- Inventors: Deepak SRIVASTAVA
- Web page: https://patentgrep.com/patent/US20140155460A1

## CPC

- C12N 15/113: Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)}
- A61P 21/00: Drugs for disorders of the muscular or neuromuscular system
- C12N 15/111: {General methods applicable to biologically active non-coding nucleic acids}
- C12N 2310/14: interfering nucleic acids [NA]
- C12N 2320/11: for the determination of target sites, i.e. of active nucleic acids
- C12N 2330/10: naturally occurring
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention describes microRNAs that regulate the differentiation, proliferation and death of cardiac and skeletal muscles cells. These molecules represent unique targets in the developmental pathways of muscle cells. They also can be used as active agents to induce differentiation in progenitor cells, and their down-regulation permits the maintenance and expansion of progenitor cell populations.

## Claims

### Claim at position 1

-30. (canceled)

### Claim 31

A method of modulating the proliferation, differentiation or death of a muscle cell in vivo comprising contacting said cell with an antagonist of miR-1-1 or miR-1-2 function or expression.

### Claim 32 (depends on 31)

The method of claim 31, wherein said muscle cell is a muscle progenitor cell.

### Claim at position 4

-34. (canceled)

### Claim 35 (depends on 31)

The method of claim 31, wherein said antagonist is a peptide, protein, DNA, RNA, or small molecule.

### Claim 36

A method of inhibiting differentiation of a muscle cell progenitor in vivo comprising inhibiting the function or expression of one or more of miR-1-1 or miR-1-2.

### Claim 37 (depends on 36)

The method of claim 36, comprising inhibiting the function of miR-1-1.

### Claim 38 (depends on 36)

The method of claim 36, comprising inhibiting the function of miR-1-2.

### Claim 39 (depends on 36)

The method of claim 36, further comprising inhibiting the function of miR-133a2.

### Claim at position 10

-46. (canceled)

### Claim 47 (depends on 36)

The method of claim 36, wherein said cell is a human cell.

### Claim at position 12

-52. (canceled)

### Claim 53 (depends on 36)

The method of claim 36, wherein said cell is a cardiac muscle cell.

### Claim 54 (depends on 36)

The method of claim 36, wherein said cell is a skeletal muscle cell.

### Claim at position 15

55-64. (canceled)

### Claim 65 (depends on 31)

The method of claim 31, wherein said cell is a human cell.

### Claim 66 (depends on 31)

The method of claim 31, wherein said cell is a cardiac muscle cell.

### Claim 67 (depends on 31)

The method of claim 31, wherein said cell is a skeletal muscle cell.

### Claim 68 (depends on 31)

The method of claim 31, wherein said cell is a stem cell.

### Claim 69 (depends on 36)

The method of claim 36, wherein said cell is a stem cell.

### Claim 70 (depends on 36)

The method of claim 36, wherein said antagonist is a peptide, protein, DNA, RNA, or small molecule.

### Claim 71 (depends on 31)

The method of claim 31, wherein said antagonist is an antisense oligonucleotide.

### Claim 72 (depends on 36)

The method of claim 36, wherein said antagonist is an antisense oligonucleotide.