# US 2014/0155318 A1 · Peptides and compositions for prevention of cell adhesion and methods of using same

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- Number: US 2014/0155318 A1
- Document ID: US20140155318A1
- Status: Published application
- Kind: A1
- Application number: 14/016,480
- Filed: 2013-09-03
- Published: 2014-06-05
- Earliest claimed: 2008-12-29
- Assignees: Tel HaShomer Medical Research Infrastructure and Services Ltd.
- Applicants: Tel Hashomer Medical Research, Infrastructure and Services Ltd.
- Inventors: Amir ZLOTKIN
- Web page: https://patentgrep.com/patent/US20140155318A1

## CPC

- C07K 14/435: from animals; from humans
- A61P 31/00: Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P 31/04: Antibacterial agents
- A61P 31/10: Antimycotics
- A61P 33/02: Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
- C02F 1/441: {by reverse osmosis}
- C02F 1/50: by addition or application of a germicide or by oligodynamic treatment {(C02F1/4606, C02F1/467, C02F1/76 take precedence)}
- C07K 7/06: having 5 to 11 amino acids
- C07K 7/08: having 12 to 20 amino acids (gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68)
- C07K 14/43595: {from coelenteratae, e.g. medusae}
- C02F 2303/04: Disinfection
- C02F 2303/20: Prevention of biofouling
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes)

## Abstract

Compositions comprising an isolated peptide, which may for example optionally comprise a sequence selected from the group consisting of FDYDWY (SEQ ID NO: 2), SFSQNKSVHSFDYDWYNVSDQADLKN (SEQ ID NO: 3) or CSFSQNKSVHSFDYDWYNVSDQADLKNC (SEQ ID NO: 1), or any cyclized version thereof, and methods of using same, including for treatment of or prevention of formation of microbial biofilms and against adhesion of a cell to a surface.

## Claims

### Claim 1

A peptide having a sequence selected from the group consisting of FDYDWY, SFDYDWY, SFDYDWYN, HSFDYDWYN, HSFDYDWYNV, VHSFDYDWYNV, VHSFDYDWYNVS, SVHSFDYDWYNVS, SVHSFDYDWYNVSD, KSVHSFDYDWYNVSD, KSVHSFDYDWYNVSDQ, NKSVHSFDYDWYNVSDQ, NKSVHSFDYDWYNVSDQA, QNKSVHSFDYDWYNVSDQA, QNKSVHSFDYDWYNVSDQAD, SQNKSVHSFDYDWYNVSDQAD, SQNKSVHSFDYDWYNVSDQADL, FSQNKSVHSFDYDWYNVSDQADL, FSQNKSVHSFDYDWYNVSDQADLK, SFSQNKSVHSFDYDWYNVSDQADLK, SFSQNKSVHSFDYDWYNVSDQADLKN, CSFSQNKSVHSFDYDWYNVSDQADLKN, CSFSQNKSVHSFDYDWYNVSDQADLKNC, and FNFDWY or combinations thereof.

### Claim 2

A composition comprising a peptide isolated from an animal source, said peptide comprising a sequence selected from the group consisting of FDYDWY, SFDYDWY, SFDYDWYN, HSFDYDWYN, HSFDYDWYNV, VHSFDYDWYNV, VHSFDYDWYNVS, SVHSFDYDWYNVS, SVHSFDYDWYNVSD, KSVHSFDYDWYNVSD, KSVHSFDYDWYNVSDQ, NKSVHSFDYDWYNVSDQ, NKSVHSFDYDWYNVSDQA, QNKSVHSFDYDWYNVSDQA, QNKSVHSFDYDWYNVSDQAD, SQNKSVHSFDYDWYNVSDQAD, SQNKSVHSFDYDWYNVSDQADL, FSQNKSVHSFDYDWYNVSDQADL, FSQNKSVHSFDYDWYNVSDQADLK, SFSQNKSVHSFDYDWYNVSDQADLK, SFSQNKSVHSFDYDWYNVSDQADLKN, CSFSQNKSVHSFDYDWYNVSDQADLKN, CSFSQNKSVHSFDYDWYNVSDQADLKNC, and FNFDWY, or a combination thereof.

### Claim 3

A method of preventing adhesion of a single cell organism to a surface, the method comprising contacting the cell with a composition comprising a peptide isolated from an animal source, said peptide comprising a sequence selected from the group consisting FDYDWY, SFSQNKSVHSFDYDWYNVSDQADLKN or CSFSQNKSVHSFDYDWYNVSDQADLKNC.

### Claim 4 (depends on 2)

The peptide- or composition of claim 2, wherein said peptide is devoid of cytotoxic or cytostatic activity.

### Claim 5 (depends on 2)

The peptide or composition of claim 2, wherein said peptide is resistant to lyophilization.

### Claim 6 (depends on 2)

The peptide or composition, of claim 2, wherein said peptide is further capable of inhibiting aggregation of cells.

### Claim 7 (depends on 3)

The method of claim 3, wherein said single cell organism is comprised in a biofilm.

### Claim 8 (depends on 3)

The method of claim 3, wherein said single cell organism is selected from the group consisting of a bacteria, a fungus, a protozoa and an archaea.

### Claim 9 (depends on 8)

The method of claim 8, wherein said fungus comprises a yeast.

### Claim 10 (depends on 3)

The method of claim 3, wherein said surface is selected from the group consisting of a fabric, a fiber, a foam, a film, a concrete, a masonry, a glass, a metal and a plastic.

### Claim 11 (depends on 3)

The method of claim 3, wherein said animal is a vertebrate.

### Claim 12 (depends on 11)

The method of claim 11, wherein said animal is selected from the group consisting of a fish, an amphibian, a bird, a reptile and a mammal.

### Claim 13 (depends on 12)

The method of claim 12, wherein said mammal is a human.

### Claim 14 (depends on 2)

A medical device comprising the peptide or composition of claim 2 attached thereto.

### Claim 15 (depends on 14)

The medical device of claim 14, wherein the medical device is an intracorporeal device.

### Claim 16 (depends on 15)

The medical device of claim 15, wherein the medical device is an extracorporeal device.

### Claim 17 (depends on 2)

A method of treating water to prevent or reduce biofilm formation or fouling of a filter, comprising treating the water with a peptides, or composition of claim 2.

### Claim 18 (depends on 2)

A method of preventing or reducing biofilm formation in a fluid medium, comprising treating the fluid medium with a peptide, or composition of claim 2.

### Claim 19 (depends on 17)

The method of claim 17, wherein said treated water or fluid medium is applied to a reverse osmosis filter.