# US 2014/0155296 A1 · Methods and compositions for improved cattle longevity and milk production > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0155296 A1 - Document ID: US20140155296A1 - Status: Published application - Kind: A1 - Application number: 14/040,757 - Filed: 2013-09-30 - Published: 2014-06-05 - Earliest claimed: 2012-12-03 - Assignees: Wisconsin Alumni Research Foundation - Applicants: Wisconsin Alumni Research Foundation - Inventors: Hasan Khatib; Wen Huang - Web page: https://patentgrep.com/patent/US20140155296A1 ## CPC - C12Q 1/6888: for detection or identification of organisms - A61D 19/02: for artificial insemination - C12Q 1/6876: Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes - C12Q 1/6883: for diseases caused by alterations of genetic material - C12Q 2600/156: Polymorphic or mutational markers - C12Q 2600/172: Haplotypes - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - A61D: VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS ## Abstract A single nucleotide polymorphic site at position 10793 of the bovine POU1F1 gene is associated with improved longevity and milk product traits. Disclosed are nucleic acid molecules, kits, methods of genotyping and marker assisted bovine breeding methods. ## Claims ### Claim 1 An isolated nucleic acid molecule comprising a polymorphic site at position 10793 and at least 15 contiguous bases of SEQ ID NO: 1 adjacent to the polymorphic site, wherein the nucleic acid molecule comprises an adenine base at position 10793, or a nucleic acid molecule that is fully complementary to the nucleic acid molecule. ### Claim 2 (depends on 1) A nucleic acid molecule according to claim 1, which comprises at least 17 contiguous bases of SEQ ID NO: 1 adjacent to the polymorphic site. ### Claim 3 (depends on 1) A nucleic acid molecule according to claim 1, which comprises at least 20 contiguous bases of SEQ ID NO: 1 adjacent to the polymorphic site. ### Claim 4 (depends on 1) An isolated nucleic acid molecule according to claim 1, which comprises not more than 150 nt. ### Claim 5 (depends on 1) An isolated nucleic acid molecule according to claim 1, which comprises not more than 100 nt. ### Claim 6 (depends on 1) An isolated nucleic acid molecule according to claim 1, which comprises not more than 50 nt. ### Claim 7 (depends on 1) A nucleic acid molecule according to claim 1, wherein the polymorphic site is within 4 nucleotides of the center of the nucleic acid molecule. ### Claim 8 (depends on 7) A nucleic acid molecule according to claim 7, wherein the polymorphic site is at the center of the nucleic acid molecule. ### Claim 9 (depends on 1) A nucleic acid molecule according to claim 1, wherein the polymorphic site is at the 3′-end of the nucleic acid molecule. ### Claim 10 (depends on 8) An array of nucleic acid molecules comprising at least two nucleic acid molecules according to claim 8. ### Claim 11 (depends on 1) A kit comprising a nucleic acid molecule of claim 1, and a suitable container. ### Claim 12 A method for detecting single nucleotide polymorphism (SNP) in bovine POU1F1 gene, wherein the PI gene have a nucleic acid sequence of SEQ ID NO: 1, the method comprising determining the identity of a nucleotide at position 10793, and comparing the identity to the nucleotide identity at a corresponding position of SEQ ID NO: 1. ### Claim 13 (depends on 12) A method for genotyping a bovine cell, comprising obtaining a nucleic acid sample from said cell and determining the identity of the nucleotide of a position of 10793 of the bovine POU1F1 gene according to claim 12. ### Claim 14 (depends on 13) A method according to claim 13, wherein the bovine cell is an adult cell, an embryo cell, a sperm, an egg, a fertilized egg, or a zygote. ### Claim 15 (depends on 13) method according to claim 13, wherein the identity of the nucleotide is determined by sequencing the PI gene, or a relevant fragment thereof, isolated from the cell. ### Claim 16 (depends on 16) A method according to claim 16, wherein the gene or a relevant fragment thereof is isolated from the cell via amplification by the polymerase chain reaction (PCR) of genomic DNA of the cell, or by RT-PCR of the mRNA of the cell. ### Claim 17 (depends on 15) A method according to claim 15, wherein both copies of the gene in the cell are genotyped. ### Claim 18 (depends on 13) A method for progeny testing of cattle, the method comprising collecting a nucleic acid sample from said progeny, and genotyping said nucleic sample according to claim 13. ### Claim 19 (depends on 15) A method for selectively breeding of cattle using a multiple ovulation and embryo transfer procedure (MOET), the method comprising superovulating a female animal, collecting eggs from said superovulated female, in vitro fertilizing said eggs from a suitable male animal, implanting said fertilized eggs into other females allowing for an embryo to develop, and genotyping said developing embryo according to claim 15, and terminating pregnancy if said developing embryo does not have A at position 10793 of the POU1F1 gene. ### Claim 20 (depends on 19) A method according to claim 19, wherein pregnancy is terminated if the embryo is not homozygously A at position 10793 of the POU1F1 gene. ### Claim 21 A method for selectively breeding dairy cattles, comprising selecting a bull that is homozygously A at position 10793 of the POU1F1 gene and using its semen for fertilizing a female animal. ### Claim 22 (depends on 21) A method according to claim 21, wherein the female animal is in vitro fertilized. ### Claim 23 (depends on 21) A method according to claim 21, wherein MOET procedure is used. ### Claim 24 (depends on 21) A method according to claim 21, wherein said female animal is also homozygously A at position 10793 of the POU1F1 gene. ### Claim 25 (depends on 13) A method for testing a dairy cattle for its longevity or milk production trait, or both, comprising genotyping its cells according to claim 13, wherein a cattle homozygously having A at position 10793 of the POU1F1 gene indicates that the cattle has desirable milk production trait.