# US 2014/0154791 A1 · Processing microtitre plates for covalent immobilization chemistries

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- Number: US 2014/0154791 A1
- Document ID: US20140154791A1
- Status: Published application
- Kind: A1
- Application number: 14/175,182
- Filed: 2014-02-07
- Published: 2014-06-05
- Earliest claimed: 2009-11-17
- Assignees: The Government of the United States of America, as represented by the Secretary of the Navy
- Inventors: Stella H. North; Evgeniya H. Lock; Scott G. Walton; Chris Rowe Taitt
- Web page: https://patentgrep.com/patent/US20140154791A1

## CPC

- B01J 19/0046: {Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays (synthesis methods per se C40B50/00)}
- B29C 59/14: by plasma treatment {(plasma tubes per se H01J)}
- C08J 7/123: {Treatment by wave energy or particle radiation (C08J7/18 takes precedence)}
- C08J 7/16: with polymerisable compounds
- G01N 33/54353: {with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393)}
- B01J 2219/00587: High throughput processes
- B01J 2219/00596: Solid-phase processes
- B01J 2219/00711: Light-directed synthesis
- B01J 2219/00725: Peptides
- C08J 2325/06: Polystyrene
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B29C: SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING (making preforms B29B11/00; making laminated products by combining previously unconnected layers which become one product whose layers will remain together B32B37/00 - B32B41/00)
- C08J: WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H (working, e.g. shaping, of plastics B29)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Disclosed herein is a method of: treating an organic polymer with an electron beam-generated plasma; exposing the treated polymer to air or an oxygen- and hydrogen-containing gas, generating hydroxyl groups on the surface of the polymer; reacting the surface with an organosilane compound having a chloro, fluoro, or alkoxy group and a functional or reactive group that is less reactive with the surface than the chloro, fluoro, or alkoxy group; and covalently immobilizing a biomolecule to the functional or reactive group or a reaction product thereof.

## Claims

### Claim 1

An article make by a method comprising:
treating a microtitre plate comprising an organic polymer and comprising a plurality of wells with an electron beam-generated plasma;
exposing the treated polymer to air or an oxygen- and hydrogen-containing gas;
wherein the treating and exposing generates hydroxyl groups on the surface of the polymer;
reacting the surface with an organosilane compound having a chloro, fluoro, or alkoxy group and a functional or reactive group that is less reactive with the surface than the chloro, fluoro, or alkoxy group; and
covalently immobilizing a biomolecule to the functional or reactive group or a reaction product thereof.

### Claim 2

An article comprising:
a polymeric microtitre plate comprising a plurality of wells; and
two or more different organosilane compounds having a functional or reactive group and bound to the surfaces of the wells;
wherein at least two of the wells have different organosilane compounds bound therein.

### Claim 3 (depends on 2)

The article of claim 2, wherein one or more biomolecules are covalently immobilized to the functional or reactive group or a reaction product thereof.

### Claim 4

An article comprising:
a polymeric microtitre plate comprising a plurality of wells; and
one or more organosilane compounds bound to the surfaces of the wells;
wherein the organosilane compounds in at least two different wells are each reacted with a different crosslinker.

### Claim 5 (depends on 4)

The article of claim 4, wherein one or more biomolecules are covalently immobilized to the crosslinkers.

### Claim 6

A method comprising:
treating an organic polymer microtitre plate with an electron beam-generated plasma;
exposing the treated plate to air or an oxygen- and hydrogen-containing gas;
wherein the treating and exposing generates hydroxyl groups on the surface of the plate.