# US 2014/0154787 A1 · Method and apparatus for biological sample analysis

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- Number: US 2014/0154787 A1
- Document ID: US20140154787A1
- Status: Published application
- Kind: A1
- Application number: 13/915,476
- Filed: 2013-06-11
- Published: 2014-06-05
- Earliest claimed: 2006-11-30
- Assignees: Fluidigm Corporation
- Applicants: Fluidigm Corporation
- Inventors: Gang Sun; Ramesh Ramakrishnan; Robert C. Jones
- Web page: https://patentgrep.com/patent/US20140154787A1

## CPC

- C12Q 1/686: Polymerase chain reaction [PCR]
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

A method of adjusting amplification curves in a PCR experiment includes receiving a plurality of amplification curves for a sample and computing a first parameter for each of the plurality of amplification curves. The method also includes computing a second parameter for each of the plurality of amplification curves and computing a third parameter using at least a portion of the first or second parameters. The method further includes computing an offset for each of the plurality of amplification curves. The offset is a function of the first parameter and the third parameter. Moreover, the method includes adjusting at least one of the plurality of amplification curves by subtracting the offset.

## Claims

### Claim 1

A system for characterizing biological processes, the system comprising:
an imaging system adapted to capture a series of images of a microfluidic device, wherein each of the plurality of images includes a plurality of sample chambers;
a processor adapted to:
compute a plurality of amplification curves for each of the plurality of sample chambers;
compute a first parameter for each of the plurality of amplification curves;
compute a second parameter for each of the plurality of amplification curves;
compute a third parameter using at least a portion of the first or second parameters;
compute an offset for each of the plurality of amplification curves, the offset being a function of the first parameter and the third parameter; and
adjust at least one of the plurality of amplification curves by subtracting the offset; and
a memory adapted to store the plurality of adjusted amplification curves.

### Claim 2 (depends on 1)

The system of claim 1 wherein the biological processes comprise PCR.

### Claim 3 (depends on 1)

The system of claim 1 wherein the series of images comprises a series of fluorescent images.