# US 2014/0154777 A1 · Novel glucose dehydrogenase > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0154777 A1 - Document ID: US20140154777A1 - Status: Published application - Kind: A1 - Application number: 14/176,701 - Filed: 2014-02-10 - Published: 2014-06-05 - Earliest claimed: 2011-08-11 - Assignees: Toyobo Co., Ltd. - Applicants: Toyobo Co., Ltd. - Inventors: Yosuke SUMIDA; Hiroshi AIBA; Hiroshi KAWAMINAMI; Rie HIRAO; Yuu UTASHIMA; Takahide KISHIMOTO; Shusaku YANAGIDANI - Web page: https://patentgrep.com/patent/US20140154777A1 ## CPC - C12N 9/0006: {acting on CH-OH groups as donors (1.1)} - C12Q 1/54: involving glucose or galactose - C12Y 101/05: with a quinone or similar compound as acceptor (1.1.5) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - C12Y: ENZYMES ## Abstract An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity is provided. ## Claims ### Claim 1 An isolated flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity. ### Claim 2 (depends on 1) The isolated flavin-binding glucose dehydrogenase of claim 1, wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 85% or more identity to the amino acid sequence of SEQ ID NO: 1. ### Claim 3 (depends on 1) The isolated flavin-binding glucose dehydrogenase of claim 1, wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 90% or more identity to the amino acid sequence of SEQ ID NO: 1. ### Claim 4 (depends on 1) The isolated flavin-binding glucose dehydrogenase of claim 1, wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 95% or more identity to the amino acid sequence of SEQ ID NO: 1. ### Claim 5 (depends on 1) The isolated flavin-binding glucose dehydrogenase of claim 1, wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 98% or more identity to the amino acid sequence of SEQ ID NO: 1. ### Claim 6 (depends on 1) The isolate flavin-binding glucose dehydrogenase of claim 1, which further has at least one of the following characteristics (1) to (8): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 70 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 20 mM or less; (3) Substrate specificity: the reactivity to D-xylose is 1.7% or less, based on the reactivity to D-glucose taken as 100%; (4) Optimal activity temperature: 37 to 45° C.; (5) Optimal activity pH: 6.5; (6) pH stability: stable at a pH range of 5 to 7; (7) Temperature stability: stable at a temperature of 40° C. or less; and (8) Origin: the enzyme is derived from microorganisms of the genus Circinella. ### Claim 7 A flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity, wherein the polypeptide does not comprise the amino acid sequence of SEQ ID NO: 1. ### Claim 8 (depends on 7) The flavin-binding glucose dehydrogenase of claim 7, wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 85% or more identity to the amino acid sequence of SEQ ID NO: 1. ### Claim 9 (depends on 7) The flavin-binding glucose dehydrogenase of claim 7, wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 90% or more identity to the amino acid sequence of SEQ ID NO: 1. ### Claim 10 (depends on 7) The flavin-binding glucose dehydrogenase of claim 7, wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 95% or more identity to the amino acid sequence of SEQ ID NO: 1. ### Claim 11 (depends on 7) The flavin-binding glucose dehydrogenase of claim 7, wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 98% or more identity to the amino acid sequence of SEQ ID NO: 1. ### Claim 12 The flavin-binding glucose dehydrogenase of 7, wherein the recombinant expression of the DNA comprises: preparing a recombinant vector comprising a DNA encoding a polypeptide having 80% or more identity with the amino acid sequence of SEQ ID NO: 1, introducing the recombinant vector into a cell, and cultivating the cell. ### Claim 13 The flavin-binding glucose dehydrogenase of 12, wherein the cell is not Circinella simplex NBRC6412. ### Claim 14 (depends on 7) The flavin-binding glucose dehydrogenase of claim 7, which further has at least one of the following characteristics (1) to (8): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 70 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 20 mM or less; (3) Substrate specificity: the reactivity to D-xylose is 1.7% or less, based on the reactivity to D-glucose taken as 100%; (4) Optimal activity temperature: 37 to 45° C.; (5) Optimal activity pH: 6.5; (6) pH stability: stable at a pH range of 5 to 7; (7) Temperature stability: stable at a temperature of 40° C. or less; and (8) Origin: the enzyme is derived from microorganisms of the genus Circinella. ### Claim 15 A flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity, wherein the polypeptide is obtained by recombinantly expressing a DNA encoding a polypeptide having 80% or more identity with the amino acid sequence of SEQ ID NO: 1. ### Claim 16 (depends on 15) The flavin-binding glucose dehydrogenase of claim 15, wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 85% or more identity to the amino acid sequence of SEQ ID NO: 1. ### Claim 17 (depends on 15) The flavin-binding glucose dehydrogenase of claim 15, wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 90% or more identity to the amino acid sequence of SEQ ID NO: 1. ### Claim 18 (depends on 15) The flavin-binding glucose dehydrogenase of claim 15, wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 95% or more identity to the amino acid sequence of SEQ ID NO: 1. ### Claim 19 (depends on 15) The flavin-binding glucose dehydrogenase of claim 15, wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 98% or more identity to the amino acid sequence of SEQ ID NO: 1. ### Claim 20 (depends on 15) The flavin-binding glucose dehydrogenase of claim 15, which further has at least one of the following characteristics (1) to (8): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 70 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 20 mM or less; (3) Substrate specificity: the reactivity to D-xylose is 1.7% or less, based on the reactivity to D-glucose taken as 100%; (4) Optimal activity temperature: 37 to 45° C.; (5) Optimal activity pH: 6.5; (6) pH stability: stable at a pH range of 5 to 7; (7) Temperature stability: stable at a temperature of 40° C. or less; and (8) Origin: the enzyme is derived from microorganisms of the genus Circinella.