# US 2014/0154771 A1 · Cell and biofactor printable biopapers

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- Number: US 2014/0154771 A1
- Document ID: US20140154771A1
- Status: Published application
- Kind: A1
- Application number: 14/177,240
- Filed: 2014-02-11
- Published: 2014-06-05
- Earliest claimed: 2011-04-29
- Assignees: The Government of the United States of America, as represented by the Secretary of the Navy
- Inventors: Bradley R. Ringeisen; Russell Kirk Pirlo; Peter K. Wu
- Web page: https://patentgrep.com/patent/US20140154771A1

## CPC

- C12N 5/0068: {General culture methods using substrates (for specific animal cell type C12N5/06)}
- C12N 11/02: Enzymes or microbial cells immobilised on or in an organic carrier
- C12N 11/096: Polyesters; Polyamides
- C12N 11/098: formed in the presence of the enzymes or microbial cells
- C12N 2533/40: Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
- Y10T 29/49826: Assembling or joining
- Y10T 428/24273: including aperture
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

Disclosed herein is a structure having: a porous polymeric film permeated by a first extracellular matrix material; and a topcoat layer comprising a second extracellular matrix gel disposed on the film. Also disclosed herein is a method of: providing a porous polymeric film; permeating the film with a first extracellular matrix material; and applying a topcoat layer of a second extracellular matrix material to the film. Also disclosed herein is a method of: laser-machining holes through a film comprising collagen to form a web-like structure.

## Claims

### Claim 1

A method comprising:
providing a structure comprising:
a porous polymeric film permeated by a first extracellular matrix material; and
a topcoat layer comprising a second extracellular matrix gel disposed on the film; and
placing living cells on or within the topcoat layer to form a cell-seeded structure.

### Claim 2 (depends on 1)

The method of claim 1, wherein the cells are placed by a laser transfer method, laser-based printing, ink jet printing, micropen printing, syringe deposition, electrospray deposition, or a conformal printing method.

### Claim 3 (depends on 1)

The method of claim 1, further comprising:
forming one or more additional cell-seeded structures; and
stacking the cell-seeded structures to form a stacked structure having alternating layers of polymeric film and topcoat layers.

### Claim 4 (depends on 3)

The method of claim 3, further comprising:
incubating the stacked structure under conditions to induce cell differentiation or cell growth.

### Claim 5 (depends on 4)

The method of claim 4, wherein the first or second extracellular matrix material comprises a growth factor.
laser-machining holes through the film to form a web-like structure.

### Claim 6

A method comprising:
laser-machining holes through a film comprising collagen to form a web-like structure.

### Claim 7 (depends on 6)

A film made by the method of claim 6.

### Claim 8 (depends on 7)

The film of claim 7, wherein the film is no more than 50 microns thick.