# US 2014/0154768 A1 · Genes Encoding Z,Z-Farnesyl Diphosphate Synthase and A Sesquiterpene Synthase with Multiple Products and Uses Thereof

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- Number: US 2014/0154768 A1
- Document ID: US20140154768A1
- Status: Published application
- Kind: A1
- Application number: 14/143,539
- Filed: 2013-12-30
- Published: 2014-06-05
- Earliest claimed: 2007-04-03
- Applicants: Philip Morris Products S.A.
- Inventors: CHRISTOPHE SALLAUD; DENIS RONTEIN; ALAIN TISSIER
- Web page: https://patentgrep.com/patent/US20140154768A1

## CPC

- C12P 5/007: {containing one or more isoprene units, i.e. terpenes (carotenes C12P23/00)}
- C12N 9/1085: {transferring alkyl or aryl groups other than methyl groups (2.5)}
- C12N 15/52: Genes encoding for enzymes or proenzymes
- C12N 15/8243: {involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine}
- C12P 7/04: acyclic
- C12P 9/00: Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen {(phosphoglycerides, C12P7/6481)}
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to the genes involved in the biosynthetic pathway for sesquiterpenes of SB type (alpha-santalene, epi-beta-santalene, cis-alpha-bergamotene, trans-alpha-bergamotene and endo-beta-bergamotene,) and for the precursor thereof, Z,Z-farnesyl diphosphate (Z,Z-FPP), involving a Z,Z-FPP synthase and a sesquiterpene SB synthase, and to the uses thereof for producing sesquiterpeniques compounds of SB type.

## Claims

### Claim 1

A method for producing sesquiterpenes in a cell comprising:
a) introducing into said cell a construct having an expression cassette comprising a first gene encoding a Z,Z-farnesyl diphosphate synthase (zFPS) having at least 80% sequence identity to SEQ ID NO: 2 and a construct having an expression cassette comprising a second gene encoding a SB synthase (SBS) having at least 80% sequence identity to SEQ ID NO: 4; and
b) growing the transformed cell in the presence of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) under conditions suitable for the expression of said first and said second genes.

### Claim 2 (depends on 1)

The method according to claim 1, further comprising collecting said sesquiterpenes contained in said cell and/or in culture medium containing said cell.

### Claim 3

A cDNA encoding a protein having at least 80% sequence identity to SEQ ID NO: 2 or having at least 80% sequence identity to SEQ ID NO: 4.

### Claim 4

A vector comprising a nucleic acid encoding a protein having at least 80% sequence identity to SEQ ID NO: 2 or having at least 80% sequence identity to SEQ ID NO: 4.

### Claim 5 (depends on 4)

The vector according to claim 4 comprising an expression cassette, said expression cassette comprising the nucleic acid encoding a protein having at least 80% sequence identity to SEQ ID NO: 2 or having at least 80% sequence identity to SEQ ID NO: 4.