# US 2014/0154763 A1 · Method for producing ethanol using basidiomycete

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- Number: US 2014/0154763 A1
- Document ID: US20140154763A1
- Status: Published application
- Kind: A1
- Application number: 14/122,893
- Filed: 2012-03-02
- Published: 2014-06-05
- Earliest claimed: 2011-05-31
- Assignees: University of Miyazaki; National University Corporation Shizuoka University; Kyushu University, National University Corporation
- Inventors: Ichiro Kamei; Sadatoshi Meguro; Ryuichiro Kondo; Toshio Mori; Hirofumi Hirai
- Web page: https://patentgrep.com/patent/US20140154763A1

## CPC

- C12P 7/10: substrate containing cellulosic material
- C12N 1/145: Fungi isolates
- C12P 7/065: {with microorganisms other than yeasts}
- C12R 2001/645: Fungi {; Processes using fungi}
- Y02E 50/10: Biofuels, e.g. bio-diesel
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

## Abstract

An object of the present invention is to provide a means for conveniently producing ethanol with high efficiency from a carbon source derived from a plant biomass resource or the like. The present invention relates to a method for producing ethanol, comprising a step of generating ethanol by culturing basidiomycetes belonging to the genus Phlebia with a carbon source. As carbon sources, cellulose, hemicellulose, glucose, xylose, and the like or plant biomass resources containing the same can be used.

## Claims

### Claim at position 1

1-8. (canceled)

### Claim 9

A method for producing ethanol, comprising:
a pretreatment step for culturing a basidiomycete belonging to the genus Phlebia with a carbon source under aerobic conditions; and
a fermentation step for further culturing the basidiomycete with the carbon source under semi-aerobic conditions or anaerobic conditions after the pretreatment step, so as to generate ethanol.

### Claim 10 (depends on 9)

The method according to claim 9, wherein the pretreatment step and the fermentation step are performed within the same reactor.

### Claim 11 (depends on 9)

The method according to claim 9, wherein the basidiomycete belonging to the genus Phlebia is Phlebia sp. MKFC40001 (NITE BP-1099).

### Claim 12 (depends on 9)

The method according to claim 9, wherein the carbon source is a polysaccharide.

### Claim 13 (depends on 12)

The method according to claim 12, wherein the polysaccharide is in the form of a plant biomass material, crystalline cellulose, paper, pulp, or cotton linter.

### Claim 14 (depends on 9)

The method according to claim 9, wherein the carbon source is at least one type of saccharides selected from the group consisting of glucose, xylose, mannose, galactose, fructose, and arabinose.

### Claim 15 (depends on 9)

An inoculum, which is used for the method of claim 9, comprising a basidiomycete belonging to the genus Phlebia, and a carrier supporting the basidiomycete.

### Claim 16 (depends on 15)

The inoculum according to claim 15, wherein the basidiomycete belonging to the genus Phlebia is Phlebia sp. MKFC40001 (NITE BP-1099).