# US 2014/0154751 A1 · Enhanced fermentation of cellodextrins and beta-d-glucose > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0154751 A1 - Document ID: US20140154751A1 - Status: Published application - Kind: A1 - Application number: 13/980,866 - Filed: 2012-01-20 - Published: 2014-06-05 - Earliest claimed: 2011-01-21 - Assignees: The Regents of the Univeristy of California; The Board of Trustee of the University of Illinois - Inventors: James H. Doudna Cate; William T. Beeson, IV; Jonathan M. Galazka; Huimin Zhao; Sijin Li; Yong-Su Jin; Suk-Jin Ha - Web page: https://patentgrep.com/patent/US20140154751A1 ## CPC - C12P 19/12: Disaccharides - C07K 14/195: from bacteria - C07K 14/37: from fungi - C12N 9/2445: {Beta-glucosidase (3.2.1.21)} - C12N 9/90: Isomerases (5.) - C12P 7/10: substrate containing cellulosic material - C12P 19/14: produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} - C12P 19/16: produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin (non-biological hydrolysis of starch C08B30/00) - C12P 19/24: produced by the action of an isomerase, e.g. fructose - Y02E 50/10: Biofuels, e.g. bio-diesel - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION ## Abstract The present disclosure provides compositions and methods for the fermentation of cellodextrins and β-D-glucose. Host cells and recombinant polypeptides having glucose mutarotase activity are provided. Additionally, methods for improving cell growth, production of chemicals, and consumption of cellodextrins and β-D-glucose during fermentation of mixtures containing cellodextrins and β-D-glucose are provided. ## Claims ### Claim 1 A host cell comprising: a) recombinant DNA encoding one or more cellodextrin transporters; b) recombinant DNA encoding one or more polypeptides having glucose mutarotase activity; wherein the host cell consumes more molecules of cellobiose when grown in a cellobiose-containing medium than are consumed by a corresponding host cell lacking said recombinant DNA encoding one or more polypeptides having glucose mutarotase activity. ### Claim 2 (depends on 1) The host cell of claim 1, wherein the host cell further comprises recombinant DNA encoding one or more β-glucosidases. ### Claim 3 (depends on 1) The host cell of claim 1, wherein the polypeptide having glucose mutarotase activity is a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 17, 19, 21, and 23. ### Claim 4 (depends on 1) The host cell of claim 1, wherein the polypeptide having glucose mutarotase activity is a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 28-32. ### Claim 5 (depends on 1) A method of fermenting a cellobiose-containing mixture, the method comprising: a) contacting the cellobiose-containing mixture with the host cell of claim 1, and b) incubating the host cell under conditions that support fermentation. ### Claim 6 (canceled) ### Claim 7 A method of increasing production of a chemical, the method comprising: a) providing a host cell, wherein the host cell comprises: i) recombinant DNA encoding one or more cellodextrin transporters; ii) recombinant DNA encoding one or more polypeptides having glucose mutarotase activity; and b) culturing the host cell in a medium such that the recombinant DNA encoding the one or more cellodextrin transporters and the one or more polypeptides having glucose mutarotase activity are expressed, and wherein expression of the recombinant DNA encoding one or more polypeptides having glucose mutarotase activity results in increased production of the chemical by the cell as compared with a corresponding host cell lacking said recombinant DNA encoding one or more polypeptides having glucose mutarotase activity. ### Claim 8 (depends on 7) The method of claim 7, wherein the chemical is an alcohol. ### Claim 9 (depends on 8) The method of claim 8, wherein the alcohol is selected from group consisting of: ethanol, n-propanol, n-butanol, iso-butanol, 3-methyl-1-butanol, 2-methyl-1-butanol, 3-methyl-1-pentanol, and octanol. ### Claim 10 (depends on 7) The method of claim 7, wherein the chemical is a terpenoid, a polyketide, a fatty acid, a fatty acid derivative, or an organic acid. ### Claim 11 A method of increasing the growth rate of a cell, the method comprising: a) providing a host cell, wherein the host cell comprises: i) recombinant DNA encoding one or more cellodextrin transporters; ii) recombinant DNA encoding one or more polypeptides having glucose mutarotase activity; and b) culturing the host cell in a medium such that the recombinant DNA encoding the one or more cellodextrin transporters and the one or more polypeptides having glucose mutarotase activity are expressed, and wherein expression of the recombinant DNA encoding one or more polypeptides having glucose mutarotase activity results in increased growth rate of the cell as compared with a corresponding host cell lacking said recombinant DNA encoding one or more polypeptides having glucose mutarotase activity. ### Claim 12 (depends on 7) The method of claim 7, wherein the host cell further comprises recombinant DNA encoding one or more β-glucosidases. ### Claim 13 (depends on 7) The method of claim 7, wherein the polypeptide having mutarotase activity is a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 17, 19, 21, and 23. ### Claim 14 (depends on 7) The method of, claim 7, wherein the polypeptide having mutarotase activity is a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 28-32. ### Claim 15 (canceled) ### Claim 16 A method of fermenting a β-D-glucose-containing mixture, the method comprising: a) providing a host cell, wherein the host cell comprises recombinant DNA encoding one or more polypeptides having glucose mutarotase activity; and b) culturing the host cell in a medium such that the recombinant DNA encoding the one or more polypeptides having glucose mutarotase activity is expressed, and wherein expression of the recombinant DNA encoding one or more polypeptides having glucose mutarotase activity results in increased consumption of β-D-glucose-containing mixture by the cell as compared with a corresponding host cell lacking said recombinant DNA encoding one or more polypeptides having glucose mutarotase activity. ### Claim 17 (depends on 16) The method of claim 16, wherein the β-D-glucose-containing mixture is obtained from the hydrolysis of cellulose. ### Claim 18 (depends on 16) The method of claim 16, wherein the polypeptide having mutarotase activity is a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 17, 19, 21, and 23. ### Claim 19 (depends on 16) The method of claim 16, wherein the polypeptide having mutarotase activity is a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 28-32. ### Claim 20 (depends on 1) The host cell of claim 1 wherein the polypeptide having glucose mutarotase activity comprises one or both amino acid sequences of SEQ ID NO: 28 and 29. ### Claim 21 (depends on 20) The host cell of claim 20, wherein the polypeptide having glucose mutarotase activity comprises the amino sequences of SEQ ID NOs: 28 and 29. ### Claim 22 (depends on 20) The host cell of claim 20, wherein the host cell further comprises recombinant DNA encoding one or more β-glucosidases.