# US 2014/0154750 A1 · Strain of bacillus subtilis and applications thereof

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- Number: US 2014/0154750 A1
- Document ID: US20140154750A1
- Status: Published application
- Kind: A1
- Application number: 13/905,460
- Filed: 2013-05-30
- Published: 2014-06-05
- Earliest claimed: 2012-11-30
- Assignees: National Central University
- Applicants: National Central University
- Inventors: SHIR-LY HUANG; SHENG-HSIN CHOU
- Web page: https://patentgrep.com/patent/US20140154750A1

## CPC

- C12P 19/14: produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase}
- C12N 1/205: Bacterial isolates
- C12P 19/02: Monosaccharides (2-ketogulonic acid C12P7/60)
- C12R 2001/125: Bacillus subtilis {; Hay bacillus; Grass bacillus}
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The present invention is directed to a strain of Bacillus subtilis and applications using the strain to raise the efficiency and/or yield of generation of glucose produced by the hydrolysis of cellulose.

## Claims

### Claim 1

A method for performing a hydrolysis of a cellulosic biomass, comprising steps of:
providing the cellulosic biomass;
providing a cellulase;
providing a strain of Bacillus subtilis SH44; and
mixing the cellulosic biomass, the cellulase and the strain of Bacillus subtilis SH44 to perform the hydrolysis.

### Claim 2 (depends on 1)

The method as claimed in claim 1, wherein the hydrolysis is performed at a reaction temperature ranged from 25° C. to 70° C.

### Claim 3 (depends on 1)

The method as claimed in claim 1, wherein the cellulosic biomass is pre-treated before being reacted in the hydrolysis.

### Claim 4 (depends on 1)

The method as claimed in claim 1, wherein the cellulase includes an exoglucanase and a beta-glucosidase.

### Claim 5 (depends on 4)

The method as claimed in claim 4, wherein the cellulase further includes an endoglucanase.

### Claim 6 (depends on 1)

The method as claimed in claim 1, wherein the hydrolysis is an enzymatic hydrolysis and generates a glucose.

### Claim 7 (depends on 1)

The method as claimed in claim 1, wherein the hydrolysis has a product, the product is a glucose and the method is used to increase at least one of a yield and a generating efficiency of the glucose.

### Claim 8

A method for cutting a cellulose, comprising steps of:
providing the cellulose;
providing a cellulase;
providing a strain of Bacillus subtilis SH44; and
mixing the cellulose, the cellulase and the strain of Bacillus subtilis SH44 to cut the cellulose.

### Claim 9 (depends on 8)

The method as claimed in claim 8, wherein the cellulase includes an exoglucanase and a beta-glucosidase and the cellulose is contained in a raw material of a special chemical or a drug.

### Claim 10

A raw material of a hydrolysis of a cellulose, comprising the cellulose and a strain of Bacillus subtilis SH44.

### Claim 11 (depends on 10)

The material as claimed in claim 10 further comprising a cellulase.

### Claim 12 (depends on 11)

The material as claimed in claim 11, wherein the cellulase includes an exoglucanase and a beta-glucosidase.

### Claim 13 (depends on 12)

The method as claimed in claim 12, wherein the cellulase further includes an endoglucanase.

### Claim 14

A strain of Bacillus subtilis SH44.

### Claim 15 (depends on 14)

A mutant of the strain of Bacillus subtilis SH44 as claimed in claim 14.

### Claim 16 (depends on 15)

The mutant as claimed in claim 15 having a characteristic of increasing at least one of a yield of a product and a reactive efficiency of a cellulosic hydrolysis.

### Claim 17 (depends on 16)

The mutant as claimed in claim 16, wherein the cellulosic hydrolysis is an enzymatic hydrolysis.

### Claim 18 (depends on 16)

The mutant as claimed in claim 16, wherein the cellulosic hydrolysis has materials of the mutant, a cellulosic biomass and a cellulase.

### Claim 19 (depends on 16)

The mutant as claimed in claim 16, wherein the cellulase includes an exoglucanase and a beta-glucosidase.

### Claim 20 (depends on 16)

The mutant as claimed in claim 16, wherein the cellulosic hydrolysis has materials of the mutant, a cellulose and a cellulase.