# US 2014/0154737 A1 · Cross-linked poly-e-lysine particles

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- Number: US 2014/0154737 A1
- Document ID: US20140154737A1
- Status: Published application
- Kind: A1
- Application number: 14/112,731
- Filed: 2012-04-20
- Published: 2014-06-05
- Earliest claimed: 2011-04-20
- Assignees: Spheritech Ltd
- Inventors: Donald Wellings
- Web page: https://patentgrep.com/patent/US20140154737A1

## CPC

- C08G 69/48: Polymers modified by chemical after-treatment
- A61L 15/32: Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin {(A61L15/225 takes precedence)}
- A61L 26/0019: {obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds}
- A61L 26/0047: {Specific proteins or polypeptides not covered by groups A61L26/0033 - A61L26/0042}
- C07H 21/00: Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07K 1/042: {characterised by the nature of the carrier}
- C08B 37/00: Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof (cellulose D21; {microbiological processes C12P})
- C08J 3/126: {Polymer particles coated by polymer, e.g. core shell structures}
- C08J 3/24: Crosslinking, e.g. vulcanising, of macromolecules (mechanical aspects B29C35/00; crosslinking agents C08K)
- C09D 177/04: Polyamides derived from alpha-amino carboxylic acids (C09D177/10 takes precedence)
- C12N 5/0018: {Culture media for cell or tissue culture (media for specific animal cell type C12N5/06)}
- C12N 11/02: Enzymes or microbial cells immobilised on or in an organic carrier
- C12N 11/06: attached to the carrier via a bridging agent
- C12N 11/096: Polyesters; Polyamides
- C12N 11/098: formed in the presence of the enzymes or microbial cells
- G01N 33/532: Production of labelled immunochemicals
- G01N 33/54313: {the carrier being characterised by its particulate form}
- G01N 33/585: {with a particulate label, e.g. coloured latex}
- A61L 2300/434: of enzymes
- C08J 2477/00: Characterised by the use of polyamides obtained by reactions forming a carboxylic amide link in the main chain; Derivatives of such polymers
- Y10T 428/249991: Synthetic resin or natural rubbers
- Y10T 428/2998: including synthetic resin or polymer
- C08G: MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- C07H: SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS (derivatives of aldonic or saccharic acids C07C, C07D; aldonic acids, saccharic acids C07C59/105, C07C59/285; cyanohydrins C07C255/16; glycals C07D; compounds of unknown constitution C07G; polysaccharides, derivatives thereof C08B; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; sugar industry C13)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C08B: POLYSACCHARIDES; DERIVATIVES THEREOF (polysaccharides containing less than six saccharide radicals attached to each other by glycosidic linkages C07H; fermentation or enzyme-using processes C12P19/00; sugar industry C13; production of cellulose D21)
- C08J: WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H (working, e.g. shaping, of plastics B29)
- C09D: COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR (cosmetics A61K; processes for applying liquids or other fluent materials to surfaces, in general, B05D; staining wood B27K5/02; glazes or vitreous enamels C03C; natural resins, French polish, drying-oils, driers, turpentine, per se, C09F; polishing compositions other than French polish, ski waxes C09G; adhesives or use of materials as adhesives C09J; materials for sealing or packing joints or covers C09K3/10; materials for stopping leaks C09K3/12; processes for the electrolytic or electrophoretic production of coatings C25D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

The invention provides a cross-linked poly-ε-lysine polymer. The poly-ε-lysine and cross linker are linked by amide bonds and may the cross linker has at least two functional groups capable of reacting with an alpha carbon amine of poly-ε-lysine. The polymer is suitably insoluble in water and other solvents and is provided in particulate form. The invention provides a particulate support comprising the cross-linked poly-ε-lysine polymer and the polymer may provide the particle itself or be coated on a particle for example silica. The polymer is useful in a wide range of applications including wound treatment, as a medical diagnostic comprising a particulate support and a functional material bound or retained by the support and solid phase synthesis of peptides, oligonucleotides, oligosaccharides, immobilisation of species, cell culturing and in chromatographic separation.

## Claims

### Claim 1

A cross-linked poly-ε-lysine polymer comprising poly-ε-lysine and a cross linker linked by amide bonds, wherein the cross linker comprises at least two functional groups capable of reacting with an alpha carbon amine of poly-ε-lysine.

### Claim at position 2

2-3. (canceled)

### Claim 4 (depends on 1)

A polymer according to claim 1, wherein the cross-linker comprises a moiety derived from a compound of formula X[CO2H]n where n is 2 or more and X is a hydrophobic or hydrophilic linking group having a molecular weight of 14 to 250 excluding any functional substituents on the linking group.

### Claim 5 (depends on 1)

A polymer according to claim 1 which is insoluble in water and other solvents.

### Claim 6 (depends on 1)

A polymer according to claim 1 in particulate form.

### Claim 7 (depends on 1)

A particulate support comprising a cross-linked poly-ε-lysine polymer according to claim 1.

### Claim 8 (depends on 7)

A particulate support according to claim 7 wherein the cross-linked poly-ε-lysine support is a spherical, ellipsoidal or irregular particle.

### Claim 9 (depends on 7)

A particulate support according to claim 7 wherein the cross-linked poly-ε-lysine support is used to coat a particle directly or indirectly.

### Claim 10 (depends on 7)

A particulate support according to claim 7, wherein the cross-linked poly-ε-lysine support is used to coat and is bound covalently to a particle.

### Claim 11 (depends on 7)

A particulate support according to claim 7, wherein the cross-linked poly-ε-lysine is used to coat an organic polymer particle.

### Claim 12 (depends on 7)

A particulate support according to claim 7, wherein the cross-linked poly-ε-lysine is used to coat an inorganic polymer particle.

### Claim 13 (depends on 7)

A particulate cross-linked poly-ε-lysine based support according to claim 7, wherein the cross-linked poly-ε-lysine is functionalised to provide a material comprising a catalyst, an initiator species for peptide synthesis, an initiator species for oligonucleotide synthesis, an initiator species for solid phase organic synthesis, a pharmaceutical active, an agrochemical active, a surface for chromatographic separation, a species to promote cell culture or differentiation, a protein or other biological macromolecule.

### Claim 14 (depends on 1, 7)

A wound treatment product comprising a polymer according to claim 1 or particulate support according to claim 7, and a component or a composition for treating a wound and/or a therapeutic agent.

### Claim 15 (depends on 7)

A medical diagnostic comprising a particulate support according to claim 7, and comprising a functional material bound or retained by the support.

### Claim 16 (depends on 15)

A medical diagnostic according to claim 15, wherein the functional material comprises an enzyme supported by the polymer.

### Claim 17

(canceled)

### Claim 18 (depends on 2)

A polymer according to claim 2, wherein the cross-linker comprises two or more carboxylic acid groups and an aliphatic chain linking the two or more groups.

### Claim 19 (depends on 3)

A polymer according to claim 3, wherein the cross-linker comprises a bis carboxylic acid having two carboxylic acid groups linked by a hydrocarbon group comprising 2 to 14 carbon atoms.

### Claim 20 (depends on 1, 7)

A process for carrying out solid phase synthesis of peptides, oligonucleotides or oligosaccharides; a solid phase extraction process; a solid phase organic chemistry process; a process for the immobilisation of a species selected from solid phase reagents, metal and other catalysts, bio-catalysts, enzymes, proteins, antibodies including polyclonal and monoclonal antibodies, whole cells and polymers; a process for cell culturing; a process for preparation of a stationary phase for chromatographic separation; comprising employing a polymer according to claim 1 or a particulate support according to claim 7 in the said process.