# US 2014/0154722 A1 · Apparatus for analyzing biomaterial > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0154722 A1 - Document ID: US20140154722A1 - Status: Published application - Kind: A1 - Application number: 13/818,919 - Filed: 2012-08-10 - Published: 2014-06-05 - Earliest claimed: 2011-08-12 - Assignees: Solidus Biosciences, Inc. - Inventors: Moo-Yeal Yeal; Suk Ho Song; Dong Woo Lee; Se Hoon Jeong; Bo Sung Ku - Web page: https://patentgrep.com/patent/US20140154722A1 ## CPC - G01N 33/5008: {for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics} - B01L 3/5085: {for multiple samples, e.g. microtitration plates} - C12M 23/12: {Well or multiwell plates (C12M25/04 takes precedence)} - C12M 25/01: {Drops} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract There is provided an apparatus for analyzing a biomaterial. The apparatus includes: a first substrate including a plurality of micro-pillars formed to protrude to a predetermined height, the biomaterial being attached to one surface of the micro-pillar; a second substrate including a plurality of micro-wells, the micro-pillars being insertable into the micro-wells when the first substrate-and the second substrate are combined with each other; and at least one spacer disposed between the first substrate and the second substrate when the first substrate and the second substrate are combined with each other. ## Claims ### Claim 1 An apparatus for analyzing a biomaterial, the apparatus comprising: a first substrate comprising a plurality of micro-pillars formed on a surface of the substrate, wherein said micropillars protrude from said surface to a predetermined height, and wherein a biomaterial is attached to a surface of the micro-pillar; a second substrate comprising a plurality of micro-wells, the micro-pillars being insertable into the micro-wells when the first substrate and the second substrate are combined with each other; and at least one spacer disposed between the first substrate and the second substrate when the first substrate and the second substrate are combined with each other. ### Claim 2 (depends on 1) The apparatus of claim 1, wherein the one spacer is formed on the surface of the second substrate. ### Claim 3 (depends on 2) The apparatus of claim 2, wherein the spacer is surface-contacted or point-contacted with the first substrate. ### Claim 4 (depends on 1, 2, 3) The apparatus of any one of claims 1 to 3, wherein the first substrate further comprises a penetration hole formed between the micro-pillars thereof. ### Claim 5 (depends on 1, 2, 3, 4) The apparatus of any one of claims 1 to 4, wherein the spacer is formed at one of the ends of the first substrate or the second substrate. ### Claim 6 (depends on 1) The apparatus of claim 1, wherein the spacer is formed on the second substrate, and the first substrate further comprises a penetration hole formed between the micro-pillars thereof. ### Claim 7 (depends on 1) The apparatus of claim 1, wherein the spacer is formed on the second substrate, and wherein the first substrate further comprises a guide groove into which a portion of the spacer is inserted, the guide groove being formed at a position of the first substrate corresponding to the spacer. ### Claim 8 (depends on 7) The apparatus of claim 7, wherein the guide groove has a width that varies over the depth of groove wherein the width of one region of the guide groove is equal to the width of the spacer such that only the portion of the at least one spacer is inserted into the guide groove. ### Claim 9 (depends on 7) The apparatus of claim 7, wherein the spacer has a width that varies over the height wherein the width of one region of the spacer is equal to the width of the guide groove such that only the portion of the at least one spacer is inserted into the guide groove. ### Claim 10 (depends on 7) The apparatus of claim 7, wherein the guide groove has a depth smaller than the height of the at least one spacer. ### Claim 11 (depends on 7) The apparatus of claim 7, wherein the guide groove is formed to penetrate the first substrate. ### Claim 12 (depends on 7) The apparatus of claim 7, wherein the width of the guide groove and the width of the spacer vary in a height or depth direction thereof, the width of one region of the guide groove being equal to the width of one region of the at least one spacer such that only the portion of the at least one spacer is inserted into the guide groove. ### Claim 13 (depends on 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12) The apparatus of any one of claims 1 to 12, wherein the biomaterial is a cell. ### Claim 14 (depends on 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13) The apparatus of any one of claims 1 to 13, wherein the biomaterial is encapsulated in a three-dimensional matrix material. ### Claim 15 (depends on 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14) The apparatus of any one of claims 1 to 14, wherein the microwell comprises a reagent capable of interacting with the biomaterial. ### Claim 16 (depends on 15) The apparatus of claim 15, wherein the reagent is selected from the group consisting of an enzyme, a small molecule, a biopolymer or a combination thereof. ### Claim 17 (depends on 16) The apparatus of claim 16, wherein the enzyme is a cytochrome P450 enzyme. ### Claim 18 A method for analyzing a biomaterial, the method comprising: combining a first substrate comprising a biomaterial attached to a surface thereof with a second substrate comprising a reagent in micro-wells such that the biomaterial is brought into contact with the reagent, wherein at least one spacer is disposed between the first substrate and the second substrate when the first substrate and the second substrate are combined with each other; wherein the first substrate comprises a plurality of micro-pillars formed on a surface of the substrate, wherein said micropillars protrude from said surface to a predetermined height, and wherein a biomaterial is attached to a surface of the micro-pillar; and wherein the second substrate comprises a plurality of micro-wells, the micro-pillars being insertable into the micro-wells when the first substrate and the second substrate are combined with each other; and wherein said method further comprising detecting a reaction between the biomaterial and the reagent. ### Claim 19 (depends on 18) The method of claim 18, wherein the first substrate further comprises a penetration hole formed between the micro-pillars thereof. ### Claim 20 (depends on 18, 19) The method of any one of claims 18 to 19, wherein the spacer is formed at one of the ends of the first substrate or the second substrate. ### Claim 21 (depends on 18, 19, 20) The method of claims 18 to 20, wherein the first substrate further comprises a guide groove into which a portion of the spacer is inserted. ### Claim 22 (depends on 18, 19, 20, 21) The method of any one of claims 18 to 21, wherein the biomaterial is encapsulated in a three-dimensional matrix material. ### Claim 23 (depends on 18, 19, 20, 21, 22) The method of any one of claims 18 to 22, wherein the wherein the reagent is selected from the group consisting of an enzyme, a small molecule, a biopolymer or a combination thereof ### Claim 24 (depends on 23) The method of claim 23, wherein the enzyme is a cytochrome P450 enzyme.