# US 2014/0154699 A1 · Monoclonal Antibodies which Specifically Recognize Human Liver-Carboxylesterase 1, Hybridoma Cell Lines which Produce Monoclonal Antibodies, and Uses Thereof

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2014/0154699 A1
- Document ID: US20140154699A1
- Status: Published application
- Kind: A1
- Application number: 14/112,411
- Filed: 2012-04-17
- Published: 2014-06-05
- Earliest claimed: 2011-04-18
- Assignees: Industry-Academic Cooperation Foundation, Yonsei University
- Inventors: Young Ki Paik; Keun Na
- Web page: https://patentgrep.com/patent/US20140154699A1

## CPC

- G01N 33/573: for enzymes or isoenzymes
- C07K 16/40: against enzymes
- G01N 33/57525: of the liver or pancreas
- C07K 2317/34: Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
- G01N 2333/918: Carboxylic ester hydrolases (3.1.1)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present invention relates to monoclonal antibodies which specifically recognize human liver-carboxylesterase 1, hybridoma cell lines which produce monoclonal antibodies, and uses thereof. The invention can be used for the objective analysis of the expression amount of human liver-carboxylesterase 1 in tissues and blood, and the like, by being specifically bound to the human liver-carboxylesterase 1. Therefore, liver cancer can be conveniently and quickly diagnosed from urine or blood.

## Claims

### Claim 1

A monoclonal antibody specifically binding to a human liver-carboxylesterase 1 protein, which is produced by a hybridoma deposited under Accession No. KCLRF-BP-00282.

### Claim 2 (depends on 1)

The monoclonal antibody according to claim 1, which recognizes an epitope sequence of amino acids 554 to 567 at the C-terminal of the human liver-carboxylesterase 1 protein.

### Claim 3 (depends on 1)

The monoclonal antibody according to claim 1, which is an immunoglobulin isotype of IgG1.

### Claim 4 (depends on 1)

A hybridoma cell deposited under Accession No. KCLRF-BP-00282, which produces the monoclonal antibody of claim 1.

### Claim 5 (depends on 1)

A composition for isolating and purifying a human liver-carboxylesterase 1 protein, comprising the monoclonal antibody of claim 1.

### Claim 6 (depends on 1)

A composition for detecting a human liver-carboxylesterase 1, comprising the monoclonal antibody of claim 1.

### Claim 7 (depends on 1)

A method of detecting a concentration of a human liver-carboxylesterase 1 in the urine or blood, comprising detecting the formation of an antigen-antibody complex by contacting the monoclonal antibody of claim 1 with a sample.

### Claim 8 (depends on 1)

A kit for diagnosing liver cancer, comprising the monoclonal antibody of claim 1.