# US 2014/0154685 A1 · Method and kit for measuring micro-rna in body fluids

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- Number: US 2014/0154685 A1
- Document ID: US20140154685A1
- Status: Published application
- Kind: A1
- Application number: 13/692,477
- Filed: 2012-12-03
- Published: 2014-06-05
- Earliest claimed: 2012-12-03
- Assignees: Rosalind Franklin University of Medicine and Science
- Inventors: Dominik M. DUELLI
- Web page: https://patentgrep.com/patent/US20140154685A1

## CPC

- C12Q 1/6806: Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention provides a kit for measuring the content of micro-RNA in a human blood sample including a blood collection tube containing at least 1 μg NaF and 0.8 μg KOx; and providing a set of instructions for collecting a human blood sample in the blood collection tube.

## Claims

### Claim 1

A kit for measuring the content of micro-RNA in a human blood sample comprising:
a blood collection tube containing at least 1 μg/μl NaF and 0.8 μg/μl KOx; and
a set of instructions for collecting a human blood sample in the blood collection tube.

### Claim 2 (depends on 1)

The kit of claim 1 wherein the blood collection tube is free from heparin.

### Claim 3

A kit for measuring the content of micro-RNA in a human blood sample comprising:
a blood collection tube;
a quantity of NaF and KOx for adding to the blood collection tube so that the collection tube has at least 1 μg/μl NaF and 0.8 μg/μl KOx; and
a set of instructions for collecting human blood sample in the collection tube.

### Claim 4 (depends on 3)

The kit of claim 3 further comprising:
a quantity of heparinase I for adding to the blood collection tube.

### Claim 5

A method for collecting blood from a human subject comprising the steps of:
providing a sample of human blood in a blood collection tube;
adding a quantity of NaF and KOx to the blood collection tube so that the blood collection tube has at least 1 μg/μl NaF and 0.8 μg/μl KOx;
extracting miRNA from the blood collection tube; and
quantifying the miRNA in the sample.

### Claim 6 (depends on 5)

The method of claim 5 wherein the human blood has a volume of 5 ml.

### Claim 7 (depends on 5)

The method of claim 5 wherein the blood sample is whole blood, serum or plasma.

### Claim 8 (depends on 7)

The method of claim 7 wherein the step of quantifying the miRNA is through a polymerase chain reaction procedure.

### Claim 9 (depends on 8)

The method of claim 8 wherein the polymerase chain reaction procedure utilizes Hemo KlenTaq and an intact Taq polymerase.

### Claim 10 (depends on 5)

The method of claim 5 further comprising the step of adding heparinase Ito the collection tube.