# US 2014/0154669 A1 · Control Nucleic Acids for Multiple Parameters

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2014/0154669 A1
- Document ID: US20140154669A1
- Status: Published application
- Kind: A1
- Application number: 14/079,155
- Filed: 2013-11-13
- Published: 2014-06-05
- Earliest claimed: 2010-07-29
- Assignees: Roche Molecular Systems, Inc.
- Applicants: Roche Molecular Systems, Inc.
- Inventors: Meike Eickhoff; Ellen H. Fiss; Joachim Glaubitz; Niclas Hitziger; Stephen G. Will; Dirk Zimmermann
- Web page: https://patentgrep.com/patent/US20140154669A1

## CPC

- C12Q 1/6851: Quantitative amplification
- C12Q 1/703: {Viruses associated with AIDS}
- C12Q 1/706: {for hepatitis}
- C12Q 1/707: {non-A, non-B Hepatitis, excluding hepatitis D}
- C12Q 1/708: {for papilloma}
- C12Q 1/6883: for diseases caused by alterations of genetic material
- C12Q 2600/156: Polymorphic or mutational markers
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

Methods are provided for the amplification of at least a first and a second target nucleic acid that may be present in at least one fluid sample using an internal control nucleic acid for qualitative and/or quantitative purposes.

## Claims

### Claim at position 1

1-16. (canceled)

### Claim 17

A method for controlled amplification of at least a first and a second target nucleic acid from a plurality of biological samples, comprising:
a) adding an internal control nucleic acid to each of the plurality of biological samples in a number of reaction vessels corresponding to the number of biological samples, wherein the internal control nucleic acid is identical for each of the plurality of biological samples, and wherein the sequence of the internal control nucleic acid is different from the sequences of the target nucleic acids;
b) purifying target nucleic acids from the plurality of biological samples and the internal control nucleic acid simultaneously in the reaction vessels;
c) adding amplification reagents to the reaction vessels,
wherein the amplification reagents for the first target nucleic acid comprise at least one set of primers specific for the first target nucleic acid and at least one set of primers specific for the internal control nucleic acid, and the amplification reagents for the second target nucleic acid comprise at least one set of primers specific for the second target nucleic acid and at least one set of primers specific for the internal control nucleic acid, and
wherein the primers specific for the internal control nucleic acid are different from the primers specific for the first and second target nucleic acids;
d) incubating the reaction vessels simultaneously under identical conditions sufficient for amplification to occur;
e) detecting and measuring signals generated by the amplification;
wherein the signals generated by the amplification of the internal control nucleic acid are indicative of an amplification occurring in the reaction vessels even in the absence of signals generated by the amplification of the at least first and second target nucleic acids.

### Claim 18 (depends on 17)

The method of claim 17, further comprising:
f) determining the quantity of the at least first and second target nucleic acid.

### Claim 19 (depends on 17)

The method of claim 17, wherein the internal control nucleic acid is DNA.

### Claim 20 (depends on 17)

The method of claim 17, wherein the internal control nucleic acid is RNA.

### Claim 21 (depends on 17)

The method of claim 17, wherein the internal control nucleic acid is an armored nucleic acid.

### Claim 22 (depends on 17)

The method of claim 17, wherein the sequence of the internal control nucleic acid is derived from a plant genome.

### Claim 23 (depends on 17)

The method of claim 17, wherein the sequence of the internal control nucleic acid is scrambled.

### Claim 24 (depends on 17)

The method of claim 17, wherein the internal control nucleic acid is selected from the group consisting of SEQ ID NOS: 45-48.

### Claim 25 (depends on 17)

The method of claim 17, wherein the amplification reagents for the first target nucleic acid further comprise at least one probe specific for the first target nucleic acid and at least one probe specific for the internal control nucleic acid, and the amplification reagents for the second target nucleic acid further comprise at least one probe specific for the second target nucleic acid and at least one probe specific for the internal control nucleic acid, and
wherein the probe specific for the internal control nucleic acid is different from the probes specific for the first and second target nucleic acids.

### Claim 26 (depends on 17)

The method of claim 17, wherein the amplification reagents further comprise a polymerase with reverse transcriptase activity.

### Claim 27 (depends on 17)

The method of claim 17, wherein a single reaction vessel comprises amplification reagents for the first and the second target nucleic acids.