# US 2014/0154516 A1 · Adhesives derived from agricultural proteins > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0154516 A1 - Document ID: US20140154516A1 - Status: Published application - Kind: A1 - Application number: 14/126,321 - Filed: 2012-06-18 - Published: 2014-06-05 - Earliest claimed: 2011-06-17 - Assignees: The Governors of the University of Alberta - Inventors: Jianping Wu; Chanchan Wang - Web page: https://patentgrep.com/patent/US20140154516A1 ## CPC - C08F 289/00: Macromolecular compounds obtained by polymerising monomers on to macromolecular compounds not provided for in groups C08F251/00 - C08F287/00 - B32B 21/08: of synthetic resin - C08H 1/00: Macromolecular products derived from proteins (food proteins A23; glue, gelatine C09H) - C08L 97/02: Lignocellulosic material, e.g. wood, straw or bagasse {(manufacture of articles made from lignocellulosic material by dry processes B27N)} - C09H 3/00: Isolation of glue or gelatine from raw materials, e.g. by extracting, by heating (gelatine for foodstuffs A23J1/10) - C09J 151/08: grafted on to macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds - C09J 189/00: Adhesives based on proteins; Adhesives based on derivatives thereof - C09J 189/04: Products derived from waste materials, e.g. horn, hoof or hair - C08F 283/04: on to polycarbonamides, polyesteramides or polyimides {(C08F283/004 takes precedence)} - Y10T 428/31515: As intermediate layer - C08F: MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS - B32B: LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM - C08H: DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS (polysaccharides C08B; natural rubber C08C; natural resins or their derivatives C09F; bituminous materials C10) - C08L: COMPOSITIONS OF MACROMOLECULAR COMPOUNDS (compositions based on polymerisable monomers C08F, C08G; artificial filaments or fibres D01F; textile treating compositions D06) - C09H: PREPARATION OF GLUE OR GELATINE - C09J: ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES (preparation of glue or gelatine C09H) - Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION ## Abstract The invention is directed to adhesives derived from animal or plant proteins, particularly canola and spent hen proteins. The adhesives are prepared by extracting the proteins by pH shifting; and either treating the extracted proteins with urea or sodium dodecyl sulfate, or polymerizing the proteins to form a protein-polymer conjugate. The invention is also directed to a wood based product comprising pieces of wood based material joined or consolidated with the adhesives. ## Claims ### Claim 1 A method for preparing an adhesive derived from animal or plant proteins, comprising the steps of: a) extracting a protein fraction from an animal or plant product by pH shifting; and b) denaturing the extracted protein fraction; or c) forming a protein-polymer conjugate with the extracted protein fraction and a polymerizable monomer. ### Claim 2 (depends on 1) The method of claim 1, wherein the animal product comprises spent hen meat. ### Claim 3 (depends on 2) The method of claim 2, wherein step (a) comprises diluting spent hen meat, followed by filtration and centrifugation to obtain a myofibrillar layer. ### Claim 4 (depends on 3) The method of claim 3, wherein the pH of the myofibrillar layer is adjusted to alkaline. ### Claim 5 (canceled) ### Claim 6 (depends on 3) The method of claim 3, wherein a protein fraction is precipitated by adjusting the pH of the middle layer to an acidic pH following an incubation period at the alkaline pH. ### Claim 7 (canceled) ### Claim 8 (depends on 6) The method of claim 6, further comprising the step of centrifuging to recover spent hen protein, followed by freeze-drying. ### Claim 9 (depends on 1) The method of claim 1, wherein the plant product is canola meal. ### Claim 10 (depends on 9) The method of claim 9, wherein step (a) comprises adjusting the pH of diluted defatted canola meal to acidic. ### Claim 11 (canceled) ### Claim 12 (depends on 10) The method of claim 10, wherein the solution is centrifuged to obtain a first precipitate. ### Claim 13 (depends on 12) The method of claim 12, further comprising the step of adjusting the pH of diluted precipitate to alkaline, followed by centrifugation to obtain a supernatant. ### Claim 14 (canceled) ### Claim 15 (depends on 13) The method of claim 13, further comprising the step of adjusting the pH of the supernatant to acidic, followed by centrifugation to obtain a second precipitate. ### Claim 16 (canceled) ### Claim 17 (depends on 17) The method of claim 17, wherein the canola protein is freeze-dried. ### Claim 18 (depends on 1) The method of claim 1, wherein in step (b), the extracted protein fraction is denatured with sodium hydroxide, urea, or sodium hydroxide sulphate. ### Claim 19 (depends on 18) The method of claim 18, wherein the extracted protein fraction is denatured with urea, having a concentration of about 1 M to about 8 M. ### Claim 20 (canceled) ### Claim 21 (depends on 1) The method of claim 1, wherein the extracted protein fraction is reacted with a polymerizable monomer to form a protein-polymer conjugate. ### Claim 22 (depends on 21) The method of claim 21, wherein the monomer is glycidyl methacrylate. ### Claim 23 (depends on 22) The method of claim 22, comprising the use of an initiator, such as ammonium persulfate. ### Claim 24 An adhesive composition comprising spent-hen or canola protein, which protein modified by reaction with a polymerizable monomer comprising a methacrylate. ### Claim 25 (canceled) ### Claim 26 (depends on 24) A wood based product comprising pieces of wood based material joined or consolidated with the adhesive of claim 24. ### Claim 27 (canceled)