# US 2014/0154426 A1 · Process for Obtaining a Film Comprised of the Incorporation of Naturally-Sourced Antimicrobial Agents in a Polymeric Structure to Develop Packages for Increasing the Shelf Life of Refrigerated Meat, Preferentially Refrigerated Fresh Salmon

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- Number: US 2014/0154426 A1
- Document ID: US20140154426A1
- Status: Published application
- Kind: A1
- Application number: 13/908,570
- Filed: 2013-06-03
- Published: 2014-06-05
- Earliest claimed: 2010-12-03
- Assignees: Universidad de Santiago de Chile
- Applicants: Universidad de Santiago de Chile
- Inventors: Abel GUARDA; Javiera RUBILAR; Maria Jose GALOTTO
- Web page: https://patentgrep.com/patent/US20140154426A1

## CPC

- C08K 5/0058: {Biocides; (macromolecular substances as carriers for biocide material A01N25/10)}
- A01N 25/28: Microcapsules {or nanocapsules}
- A01N 25/34: Shaped forms, e.g. sheets, not provided for in any other sub-group of this main group
- A01N 31/08: Oxygen or sulfur directly attached to an aromatic ring system
- A23B 4/10: Coating with a protective layer; Compositions or apparatus therefor
- A23B 4/14: Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
- A23B 4/20: Organic compounds; Microorganisms; Enzymes
- B01J 13/02: Making microcapsules or microballoons {(for medical preparations A61K9/50)}
- B65D 65/42: Applications of coated or impregnated materials
- C08J 3/20: Compounding polymers with additives, e.g. colouring
- C08J 3/223: {Packed additives}
- C08J 5/18: Manufacture of films or sheets
- C09D 105/00: Coating compositions based on polysaccharides or on their derivatives, not provided for in groups C09D101/00 or C09D103/00
- C08J 2305/12: Agar-agar; Derivatives thereof
- C08K 9/10: Encapsulated ingredients
- C08K: Use of inorganic or non-macromolecular organic substances as compounding ingredients (paints, inks, varnishes, dyes, polishes, adhesives C09)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A23B: PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B65D: CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- C08J: WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H (working, e.g. shaping, of plastics B29)
- C09D: COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR (cosmetics A61K; processes for applying liquids or other fluent materials to surfaces, in general, B05D; staining wood B27K5/02; glazes or vitreous enamels C03C; natural resins, French polish, drying-oils, driers, turpentine, per se, C09F; polishing compositions other than French polish, ski waxes C09G; adhesives or use of materials as adhesives C09J; materials for sealing or packing joints or covers C09K3/10; materials for stopping leaks C09K3/12; processes for the electrolytic or electrophoretic production of coatings C25D)

## Abstract

A process for obtaining a film comprised of the incorporation of naturally-sourced antimicrobial agents in a polymeric structure to develop packages for increasing the shelf life of refrigerated meat, preferentially refrigerated fresh salmon, wherein said process comprises the following steps: a) obtaining microcapsules of a naturally-sourced antimicrobial agent in an enveloping medium, comprising the steps of: a1) producing an aqueous phase dissolving gum arabic (Quimatic) and Tween 20 in distilled water; a2) producing an oily phase through a solution of the naturally-sourced antimicrobial agent with a mixture of thymol and carvacrol; a3) preparing an emulsion incorporating the aqueous phase onto the oily phase; b) incorporating the microcapsules into polymers, previously treating them in order to reduce their surface tension (corona treatment), the microcapsules settling over the film, and letting them dry on a stove at controlled temperature.

## Claims

### Claim 1

A process for obtaining a film comprised of the incorporation of naturally-sourced antimicrobial agents into a polymeric structure to develop packages for increasing the shelf life of refrigerated meat, preferentially refrigerated fresh salmon, wherein said process comprises the following steps:
a) obtaining microcapsules of a naturally-sourced antimicrobial agent in an enveloping medium, comprising the steps of:
a1) producing an aqueous phase dissolving gum arabic (Quimatic) and Tween 20 in distilled water;
a2) producing an oily phase through a solution of the naturally-sourced antimicrobial agent with a mixture of thymol and carvacrol;
a3) preparing an emulsion incorporation the aqueous phase onto the oily phase;
b) incorporating the microcapsules into polymers, treating them previously in order to reduce their surface tension (corona treatment), the microcapsules settling over the film, and letting them dry on a stove at controlled temperature.

### Claim 2 (depends on 1)

A process for obtaining a film, according to claim 1, wherein said aqueous phase of step a1) is obtained dissolving about 15 g gum arabic (Quimatic) with about 2 g Tween 20 in about 100 ml distilled water.

### Claim 3 (depends on 2)

A process for obtaining a film, according to claim 2, wherein said gum arabic was slowly added onto the distilled water, with continuous agitation for about 5 minutes by means of a magnetic agitator at room temperature.

### Claim 4 (depends on 3)

A process for obtaining a film, according to claim 3, wherein it comprises adjusting the solution pH to a value of 6 by adding about 10 ml Na OH.

### Claim 5 (depends on 1)

A process for obtaining a film, according to claim 1, wherein in the step a2) the concentration of thymol and carvacrol in ppm is about 1,000 thymol and about 3,000 carvacrol.

### Claim 6 (depends on 1)

A process for obtaining a film, according to claim 1, wherein in the step a2) the concentration of thymol and carvacrol in ppm is about 10,000 thymol and about 10,000 carvacrol.

### Claim 7 (depends on 1)

A process for obtaining a film, according to claim 1, wherein in the step a2) the concentration of thymol and carvacrol in ppm is about 20,000 thymol and about 20,000 carvacrol.

### Claim 8 (depends on 1)

A process for obtaining a film, according to claim 1, wherein in the step a2) the concentration of thymol and carvacrol in ppm is about 50,000 thymol and about 50,000 carvacrol.

### Claim 9 (depends on 1)

A process for obtaining a film, according to claim 1, wherein in the step a2) the concentration of thymol and carvacrol in ppm is about 100,000 thymol and about 100,000 carvacrol.

### Claim 10 (depends on 5)

A process for obtaining a film, according to claim 5, wherein in the step a2) thymol and carvacrol are dissolved in about 4.3 g soy oil.

### Claim 11 (depends on 5)

A process for obtaining a film, according to claim 5, wherein in the step a2) thymol and carvacrol are dissolved in about 4.3 g petrolatum.

### Claim 12 (depends on 10)

A process for obtaining a film, according to claim 10, wherein in the step a2) the mixture is subjected to mild manual agitation for about two minutes at room temperature.

### Claim 13 (depends on 12)

A process for obtaining a film, according to claim 12, wherein in the step a3) the homogenization is performed at an increasing speed of 0 up to about 10,000 rpm, accelerating from about 1,100 rpm about every 20 seconds for about 3 minutes at room temperature.