# US 2014/0154223 A1 · Methods of pest control > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0154223 A1 - Document ID: US20140154223A1 - Status: Published application - Kind: A1 - Application number: 14/093,684 - Filed: 2013-12-02 - Published: 2014-06-05 - Earliest claimed: 2012-12-03 - Applicants: Beijing Dabeinong Technology Group Co., Ltd.; Beijing Green Agrosino Plant Protection Technology Co., Ltd.; Beijing Dabeinong Technology Group Co., Ltd. Biotech Center - Inventors: Yuejing KANG; Jie PANG; Aihong ZHANG; Peng CHENG; Xu YANG; Lihong NIU; Zhiwei JIA; Luoxu AN; Kangle TIAN; Ziqin JIANG - Web page: https://patentgrep.com/patent/US20140154223A1 ## CPC - A01N 37/46: N-acyl derivatives - A01N 63/50: Isolated enzymes; Isolated proteins (peptides A01N37/46) - C07K 14/325: Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins - C12N 15/8286: {for insect resistance} - Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract Certain embodiments of the present invention provide a method for controlling Athetis lepigone, which comprises contacting Athetis lepigone with Cry1A protein. Aspects of the present invention can achieve control of Athetis lepigone by enabling plants to produce Cry1A protein in vivo, which can be lethal to Athetis lepigone. In still other instances, the method can control Athetis lepigone throughout the growth period of the plants and provide the plants with a full protection. Additionally, the method, in certain embodiments, can be one or more of stable, complete, simple, convenient, economical, pollution-free or residue-free. ## Claims ### Claim 1 A method for controlling Athetis lepigone, wherein the method comprises contacting Athetis lepigone with Cry1A protein. ### Claim 2 (depends on 1) The method of claim 1, wherein the Cry1A protein is Cry1Ab protein. ### Claim 3 (depends on 2) The method of claim 2, wherein the Cry1Ab protein is present in a cell that expresses the Cry1Ab protein of a plant, and Athetis lepigone contacts with the Cry1Ab protein by ingestion of the cell. ### Claim 4 (depends on 3) The method of claim 3, wherein the Cry1Ab protein is present in a transgenic plant that expresses the Cry1Ab protein, and Athetis lepigone contacts with the Cry1Ab protein by ingestion of a tissue of the transgenic plant; thereafter, the growth of Athetis lepigone is suppressed, and that eventually leads to Athetis lepigone's death and achieves controlling damage of Athetis lepigone to the plant. ### Claim 5 (depends on 4) The method of claim 4, wherein the transgenic plant is in any growth periods. ### Claim 6 (depends on 4) The method of claim 4, the tissue of the transgenic plant is roots, leaves, stems, tassels, ears, anthers or filaments. ### Claim 7 (depends on 4) The method of claim 4, wherein the control of the damage of Athetis lepigone to the plant does not depend on planting location. ### Claim 8 (depends on 4) The method of claim 4, wherein the control of the damage of Athetis lepigone to the plant does not depend on planting time. ### Claim 9 (depends on 4) The method of claim 4, wherein the plant is maize. ### Claim 10 (depends on 3) The method of claim 3, wherein prior to the step of contacting, the method comprises a step to plant a transgenic seedling that comprises a polynucleotide encoding the Cry1Ab protein. ### Claim 11 (depends on 2) The method of claim 2, wherein the amino acid sequence of the Cry1Ab protein comprises an amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2. ### Claim 12 (depends on 11) The method of claim 11, wherein the nucleotide sequence encoding the Cry1Ab protein comprises a nucleotide sequence of SEQ ID NO: 3 or SEQ ID NO: 4. ### Claim 13 A method of growth suppression of Athetis lepigone, wherein the method comprises contacting Athetis lepigone with Cry1A protein. ### Claim 14 A transgenic plant that expresses Cry1A protein. ### Claim 15 (depends on 14) A method of growth suppression of Athetis lepigone, wherein the method comprises contacting Athetis lepigone with the transgenic plant of claim 14 or tissues thereof.