# US 2014/0152888 A1 · Imaging systems with movable scan mirrors > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0152888 A1 - Document ID: US20140152888A1 - Status: Published application - Kind: A1 - Application number: 13/907,801 - Filed: 2013-05-31 - Published: 2014-06-05 - Earliest claimed: 2012-06-07 - Assignees: Complete Genomics, Inc. - Applicants: Complete Genomics, Inc. - Inventors: Bryan P. Staker; Craig E. Uhrich - Web page: https://patentgrep.com/patent/US20140152888A1 ## CPC - G02B 27/646: {compensating for small deviations, e.g. due to vibration or shake (movement of one or more optical elements for control of motion blur in cameras, projectors or printers G03B2205/0007; image stabilisation in cameras peculiar to the presence or use of an electronic image sensor H04N23/68)} - G02B 21/16: adapted for ultraviolet illumination {; Fluorescence microscopes (G02B21/0076 takes precedence)} - G02B 21/367: {providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison} - G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS ## Abstract An imaging system is provided wherein a positioning stage is translated with respect to an objective lens component and a scan mirror is repositioned while a two-dimensional image is made of a biochemical site on a substrate. In an example embodiment, an imaging system comprises a camera, an objective lens component, a positioning stage, and a scan mirror controllable by a servo system that synchronizes movement of the positioning stage and the tilting of the scan mirror so that the substrate image is maintained stable during imaging of the continuously moving positioning stage. ## Claims ### Claim 1 An imaging system comprising: a camera with a two dimensional electronic sensor; an objective lens component configured to image at least a portion of a substrate onto the two dimensional sensor of the camera to produce a two-dimensional image; a positioning stage configured to move in continuous motion the substrate in a first direction with respect to the objective lens component; and a scan mirror configured to tilt in continuous motion in coordination with the moving positioning stage, while the positioning stage moves the substrate in the first direction, in order to reflect light from the objective lens component to the camera while maintaining the substrate image stable at the electronic sensor. ### Claim 2 (depends on 1) The system of claim 1 further comprising a tube lens component positioned in an optical path between the scan mirror and the camera, wherein the scan mirror is configured to direct light from the objective lens component through the tube lens component to the sensor of the camera. ### Claim 3 (depends on 1) The system of claim 1 further comprising a first tilt plate positioned in an optical path between the scan mirror and the camera, wherein the first tilt plate is configured to cause first offset corrections to the substrate image that is acquired by the objective lens component. ### Claim 4 (depends on 3) The system of claim 3 further comprising a second tilt plate positioned in the optical path between the scan mirror and the camera, wherein the second tilt plate is configured to cause second offset corrections, in the substrate image in a second direction that is perpendicular to the first offset corrections. ### Claim 5 (depends on 1) The system of claim 1 further comprising a light source and a dichroic mirror, wherein the dichroic mirror is configured at least to: (a) reflect light from the light source to illuminate the substrate; and (b) pass through light that is acquired by the objective lens component. ### Claim 6 (depends on 1) The system of claim 1 wherein movement of the position stage and the tilt mirror are made configured by a servo mechanism. ### Claim 7 (depends on 1) The system of claim 1, wherein the camera is a CMOS camera. ### Claim 8 A method for imaging at least a portion of a substrate, the method comprising: moving a positioning stage carrying nucleic acid targets on the substrate under an objective lens component; while the substrate is moving, changing angle of a scan mirror such that the substrate image that is acquired by the objective lens component is maintained still with respect to a two-dimensional electronic sensor of a camera; and recording the substrate image through the electronic sensor while the substrate is moving. ### Claim 9 (depends on 8) The method of claim 8, further comprising: measuring the angle of the scan mirror by using an angle sensor; and using measurement from the angle sensor in changing the angle of the scan mirror. ### Claim 10 (depends on 8) The method of claim 8, further comprising tilting a first optical plate disposed in an optical path between the objective lens component and the electronic sensor of the camera, to make offset corrections in the substrate image. ### Claim 11 (depends on 10) The method of claim 10, further comprising: measuring the angle of the scan mirror to detect whether there are any nonlinearities in the motion of the scan mirror; and using the offset corrections to remove from the substrate image any nonlinearities that are detected in the motion of the scan mirror. ### Claim 12 (depends on 8) The method of claim 8, wherein the camera is one of a CMOS camera and a non-CMOS camera capable of operating in a full-frame mode. ### Claim 13 An imaging system comprising: an objective lens component that images an array chip containing biochemical materials onto a two-dimensional electronic sensor of a camera; a scan mirror, capable of angular motion, that reflects light from the objective lens component to the electronic sensor; and a positioning stage that moves the array chip with respect to the objective lens component and in coordination with the scan mirror for maintaining a stable image of the array chip at the electronic sensor during continuous movement. ### Claim 14 (depends on 13) The system of claim 13, wherein the array chip has DNA macromolecules disposed thereon and the camera is CMOS camera. ### Claim 15 (depends on 13) The system of claim 13 further comprising a tube lens component, wherein the scan mirror is configured to direct light from the objective lens component through the tube lens component to the electronic sensor. ### Claim 16 (depends on 15) The system of claim 15 further comprising a first tilt plate between the scan mirror and the camera, the first tilt plate providing first image offset corrections. ### Claim 17 (depends on 16) The system of claim 16 further comprising a second tilt plate between the scan mirror and the camera, the second tilt plate providing second image offset corrections in a direction perpendicular to the first image offset corrections. ### Claim 18 (depends on 17) The system of claim 17 further comprising an angle sensor that measures an angle of the scan mirror. ### Claim 19 (depends on 18) The system of claim 18, wherein the angle sensor comprises an optical lever. ### Claim 20 (depends on 18) The system of claim 18, wherein the angle sensor comprises a laser and a linear array detector that detects light from the laser that is reflected by the scan mirror. ### Claim 21 (depends on 15) The system of claim 15 further comprising an illumination light source and a dichroic mirror disposed to illuminate the array chip while passing the array chip image to the electronic sensor.