# US 2014/0152330 A1 · Integrated carbon nanotube field effect transistor and nanochannel for sequencing

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- Number: US 2014/0152330 A1
- Document ID: US20140152330A1
- Status: Published application
- Kind: A1
- Application number: 13/971,442
- Filed: 2013-08-20
- Published: 2014-06-05
- Earliest claimed: 2012-11-30
- Assignees: International Business Machines Corporation
- Applicants: International Business Machines Corporation
- Inventors: Ali Afzali-Ardakani; Gustavo A. Stolovitzky; Deqiang Wang
- Web page: https://patentgrep.com/patent/US20140152330A1

## CPC

- G01N 27/4146: {involving nanosized elements, e.g. nanotubes, nanowires}
- B82Y 15/00: Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
- B82Y 30/00: Nanotechnology for materials or surface science, e.g. nanocomposites
- G01N 27/02: by investigating impedance
- G01N 33/48721: {Investigating individual macromolecules, e.g. by translocation through nanopores (Coulter counters in general G01N15/12; fabrication methods for nanoscale apertures B81B1/00; sequencing of nucleic acids C12Q1/68)}
- Y10S 977/958: Of biomolecule property
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

A mechanism is provided for base recognition of an integrated transistor and nanochannel. A target molecule is forced down to a carbon nanotube a single base at a time in the nanochannel by applying a gate voltage to a top electrode, and/or a narrow thickness of the nanochannel. The nanochannel exposes an exposed portion of the carbon nanotube at a bottom wall, and the top electrode is positioned over the exposed portion. The exposed portion of the carbon nanotube is smaller than the distance between bases to only accommodate the single base at a time. The target molecule is stretched by the narrow thickness and by applying a traverse voltage across a length direction of the nanochannel. The target molecule is frictionally restricted by the narrow thickness of the nanochannel to stretch is restrictedly translocates in the length direction. Current is measured to determine an identity of the single base.

## Claims

### Claim 1

A method for base recognition in an integration of a transistor and a nano channel, the method comprising:
forcing a target molecule down to a carbon nanotube a single base at a time in the nanochannel, the target molecule being forced to the carbon nanotube by applying a gate voltage to a top electrode of the transistor, by a narrow thickness of the nanochannel, or both by applying the gate voltage to the top electrode of the transistor and by the narrow thickness of the nano channel;
wherein the nanochannel having been patterned over the carbon nanotube exposes an exposed portion of the carbon nanotube at a bottom wall of the carbon nanotube, the top electrode of the transistor positioned over the exposed portion of the carbon nanotube through the nanochannel;
wherein the exposed portion of the carbon nanotube is smaller than a separating distance between bases on the target molecule, the exposed portion of the carbon nanotube being configured to only accommodate the single base at a time;
stretching the target molecule by the narrow thickness of the nanochannel and by applying a traverse voltage across a length direction of the nanochannel between a first electrode and a second electrode at opposite ends of the nanochannel in the length direction;
wherein the target molecule is frictionally restricted by the narrow thickness of the nanochannel causing the target molecule to stretch as the target molecule restrictedly translocates in the length direction while the traverse voltage is applied;
measuring a transistor current while the single base of the target molecule is forced down to the exposed portion of the carbon nanotube in the nanochannel, the single base affecting the transistor current; and
determining an identity of the single base according to a change in the transistor current while the single base is forced down to the exposed portion of the carbon nanotube in the nanochannel.

### Claim 2 (depends on 1)

The method of claim 1, wherein when the target molecule is negatively charged the gate voltage is negative.

### Claim 3 (depends on 1)

The method of claim 1, wherein when the target molecule is positively charged the gate voltage is positive.

### Claim 4 (depends on 1)

The method of claim 1, wherein the gate voltage of the top electrode generates a downward force through the nanochannel above the exposed portion, the downward force pressing the target molecule down to touch the exposed portion of the carbon nanotube.

### Claim 5 (depends on 4)

The method of claim 4, wherein when the single base is over the carbon nanotube, the downward force presses the single base to touch the exposed portion of the carbon nanotube to sense the identity of the single base.

### Claim 6 (depends on 1)

The method of claim 1, wherein the target molecule is deoxyribonucleic acid or ribonucleic acid.

### Claim 7 (depends on 1)

The method of claim 1, wherein the nanochannel is formed of an insulating material except at the exposed portion of the carbon nanotube.

### Claim 8 (depends on 7)

The method of claim 7, wherein the nanochannel has a thickness that is less than a thickness of the insulating material.

### Claim 9 (depends on 1)

The method of claim 1, wherein a thickness of the nanochannel is substantially between 1 to 2 nanometers when the target molecule is a singe strand polynucleotide in order for the target molecule to be stretched.

### Claim 10 (depends on 1)

The method of claim 1, wherein a thickness of the nanochannel is substantially between 2 to 4 nanometers when the target molecule is a double strand polynucleotide in order for the target molecule to be stretched.

### Claim 11 (depends on 1)

The method of claim 1, wherein a thickness of the nanochannel is determined by a diameter of the target molecule to be sequenced in order for the target molecule to be frictionally restricted when translocating through the nanochannel.

### Claim 12 (depends on 1)

The method of claim 1, wherein the traverse voltage is substantially between 0 to 5 volts.

### Claim 13 (depends on 1)

The method of claim 1, wherein the gate voltage is substantially between −10 to 0 volts when the target molecule is negatively charged.

### Claim 14 (depends on 1)

The method of claim 1, wherein the gate voltage is substantially between 0 to +10 volts when the target molecule is positively charged.

### Claim 15 (depends on 1)

The method of claim 1, wherein a coating molecule is applied to the carbon nanotube to increase sensitivity of the transistor;
wherein the coating molecule increases a selectivity of bases of the target molecule.

### Claim 16 (depends on 1)

The method of claim 1, wherein the transistor comprises a source electrode and a drain electrode connected by the carbon nanotube;
wherein the transistor current flows from the source electrode to the drain electrode through the carbon nanotube.