Back to results
US 2014/0134209 A1Published application
Method for producing vaccinal viral strain of a virus of the reoviridae family
AssigneeLondon School of Hygiene and Tropical Medicine
InventorsPolly Roy, Mark Boyce
Life of this patent
Estimating the term…
Abstract
The invention relates to a method for producing a modified viral strain of a virus which is a member of the Reoviridae family and, in particular, relates to vaccinal viral strains of the Orbivirus genus.
Claims18
An isolated viral ssRNA derived from the genome of a Blue Tongue virus which is a member of the Reoviridae family wherein the ssRNA comprises a mutation which destroys the function of an essential gene, wherein the essential gene encodes VP6 protein.
A screening method for identifying essential genes of a virus which is a member of the Reoviridae family, the method comprising:
- introducing a mutation into the Reoviridae virus genome so that the function of a gene is destroyed;
- transfecting a cell with viral ssRNA derived from the virus genome and comprising the mutation;
- and
- culturing the transfected cell under suitable conditions, wherein the viral gene is not an essential gene if the virus produced after culturing the transfected cell is pathogenic.
1 more element
Dependencies are inferred from the claim text.