Methods for producing recombinant proteins

A method for producing a polyclonal cell population, said method comprising the steps of:

• a) transforming one or more host cells with at least one vector comprising at least one copy of a nucleic acid sequence that encodes a component of a polyclonal protein composition, in the presence of a retroviral integrase or rep protein, such that the at least one vector integrates into the host cell genome at a plurality of locations recognized by the retroviral integrase or rep protein;
• b) generating a cell population capable of producing the component of the polyclonal protein composition;
• and
• c) mixing the cell population and a different cell population producing a different component of the polyclonal protein composition generated by performing steps (a) and (b) to produce a polyclonal cell population capable of producing the polyclonal protein composition.

A method for producing a polyclonal cell population, said method comprising the steps of:

• a) transforming one or more host cells with at least one adeno-associated viral (AAV) vector comprising at least one copy of a nucleic acid sequence, in the presence of an AAV rep protein, such that the at least one AAV vector integrates into the host cell genome at a plurality of locations recognized by the AAV rep protein and that encodes a component of a polyclonal protein composition;
• b) generating a cell population capable of producing the component of the polyclonal protein composition;
• and
• c) mixing the cell population and a different cell population capable of producing a different component of the polyclonal protein composition generated by performing steps (a) and (b) to produce a polyclonal cell population capable of producing the polyclonal protein composition.