Sample analysis by mass cytometry
AssigneeDvs Sciences Inc.
InventorSCOTT TANNER
Life of this patent
Estimating the term…
Abstract
In a mass cytometer system, a tissue sample labeled with multiple metal tags is supported on an encoded substrate for distribution profile mapping by laser ablation. Groups of elemental ions from each plume generated by each laser pulse are detected by the mass cytometer and the data is mapped according to the encoded substrate. This configuration allows for the production of a 3-dimentional distribution profile of the multiple metal tags in the tissue sample.
Claims25
A method of sample analysis by mass cytometry comprising:
- providing a sample labeled with more than one elemental tag;
- providing an encoded substrate for supporting the sample, the encoded substrate being configured with a substrate coding;
- directing at least one laser pulse onto a location of the sample and generating a discrete plume for each of the at least one laser pulse, each of the discrete plume comprises at least one of the more than one elemental tag and the substrate coding;
- introducing each of the discrete plume into an inductively coupled plasma and generating groups of elemental ions, each of the groups of elemental ions corresponding with at least one of each of the more than one elemental tag and the substrate coding;
- detecting each of the groups of elemental ions simultaneously for each of the discrete plume;
- correlating the detected groups of elemental ions with the substrate coding;
- and
- identifying the more than one elemental tag as a function of the substrate coding.
A mass cytometer system for sample analysis comprising:
- an encoded substrate for supporting a sample, the encoded substrate being configured with a substrate coding comprising an array of codified metal compositions;
- a laser ablation system configured to generate a plume from the sample and from the substrate coding;
- and
- a mass cytometer coupled to the encoded substrate for receiving the plume, the mass cytometer having an ion source to generate groups of elemental ions from the plume and an ion detector to detect the groups of elemental ions.
A sample support for laser ablation mass cytometry comprising an encoded substrate having a surface for supporting a sample of interest, the encoded substrate being configured with a substrate coding arranged to codify the encoded substrate.
Dependencies are inferred from the claim text.