Melanocortin 1 receptor ligands and methods of use

A method selected from among:

• (a) a method of preparing a MC1R peptide ligand comprising an MC1R peptide ligand coupled to a functionality, comprising:
• providing an MC1R peptide ligand;
• and covalently bonding a moiety comprising a functionality to the C-terminus or N-terminus of the MC1R peptide ligand, wherein a MC1R peptide ligand comprising a functionality is formed;
• or
• (b) a method of delivering a moiety to cells expressing the melanocortin 1 receptor (MC1R), comprising administering an MC1R-targeted agent to the cells in vitro or in vivo, wherein the MC1R-targeted agent comprises a MC1R peptide ligand and a moiety, and wherein the MC1R peptide ligand and the moiety are covalently linked by a reaction product of a first functionality coupled to the peptide ligand and a complementary second functionality coupled to the moiety;
• or
• (c) a method of treating melanoma in a human or non-human animal subject, comprising administering an MC1R-targeted agent to the subject, wherein the moiety comprises an anti-cancer agent, wherein the MC1R-targeted agent comprises a MC1R peptide ligand and a moiety, and wherein the MC1R peptide ligand and the moiety are covalently linked by a reaction product of a first functionality coupled to the peptide ligand and a complementary second functionality coupled to the moiety;
• or
• (d) a method of imaging a melanoma tumor of a subject, comprising:
• (i) administering the MC1R peptide ligand-micelle complex to the subject, wherein the MC1R peptide ligand-micelle complex comprises an MC1R peptide ligand and a micelle comprising an inner core, outer core and hydrophilic shell, wherein the MC1R peptide ligand is linked to the shell of the micelle by a linker, wherein a contrast agent is present within the inner core of the micelle, and wherein the MC1R peptide ligand-micelle complex concentrates in the tumor, and
• (ii) observing a signal from the contrast agent by an imaging device;
• or
• (e) a method of imaging a melanoma tumor, comprising:
• (ii) providing a MC1R peptide ligand-micelle complex, wherein the MC1R peptide ligand-micelle complex comprises an MC1R peptide ligand and a micelle comprising an inner core, outer core and hydrophilic shell, wherein the MC1R peptide ligand is linked to the shell of the micelle by a linker;
• (iii) incorporating a contrast agent into the inner core of said MC1R peptide ligand micelle complex;
• (iv) administering the MC1R peptide ligand-micelle complex with the contrast agent to a human or non-human animal subject, wherein the MC1R peptide ligand-micelle complex concentrates in the tumor;
• and
• (v) observing a signal from the contrast agent by an imaging device;
• or
• (f) a method of treating melanoma tumor cells in a subject, comprising:
• administering the MC1R peptide ligand-micelle complex, to the subject, wherein the MC1R peptide ligand-micelle complex comprises an MC1R peptide ligand and a micelle comprising an inner core, outer core and hydrophilic shell, wherein the MC1R peptide ligand is linked to the shell of the micelle by a linker, wherein an anti-cancer agent is present within the inner core of the micelle, and wherein the MC1R peptide ligand-micelle complex releases the anti-cancer agent at the site of the tumor;
• or
• (g) a method of treating melanoma tumor cells in a subject, comprising:
• (i) providing a MC1R peptide ligand-micelle complex, wherein the MC1R peptide ligand-micelle complex comprises an MC1R peptide ligand and a micelle comprising an inner core, outer core and hydrophilic shell, wherein the MC1R peptide ligand is linked to the shell of the micelle by a linker;
• (ii) incorporating an anti-cancer agent into the inner core of said MC1R peptide ligand-micelle complex;
• and
• (iii) administering the MC1R peptide ligand-micelle complex with the anti-cancer agent to the subject, wherein the MC1R peptide ligand-micelle complex releases the anti-cancer agent at the site of the tumor;
• or
• (h) a method of preparing a MC1R peptide ligand-micelle complex, comprising:
• providing a MC1R peptide ligand comprising a first functionality covalently attached to a MC1R targeting peptide;
• providing a multiplicity of triblock polymer chains comprising a hydrophobic polypeptide block attached to a central crosslinkable peptide block comprising a multiplicity of crosslinkable amino acid residues attached to a water soluble polymer block, wherein a portion of the triblock polymer chains further comprise a second functionality covalently attached to the water soluble polymer block distal to the central crosslinkable testicle block, wherein the second functionality is complementary to the first functionality and wherein the triblock polymer chains self-assemble into a micelle;
• and
• combining the triblock polymer chains with the MC1R peptide ligand, and wherein the first functionality and the complementary second click functionality react to form a reaction product that covalently joins the triblock polymer to the MC1R peptide ligand to form a MC1R peptide ligand-micelle complex.

A composition selected from among:

• (a) a melanocortin 1 receptor (MC1R)-targeted agent comprising a MC1R peptide ligand;
• and a moiety, wherein the MC1R peptide ligand and the moiety are covalently linked by a reaction product of a first functionality coupled to the peptide ligand and a complementary second functionality coupled to the moiety;
• or
• (b) a pharmaceutical composition comprising the MC1-R targeted agent, and a pharmaceutically acceptable carrier, wherein the MC1-R targeted agent comprises an MC1R peptide ligand and a moiety, wherein the MC1R peptide ligand and the moiety are covalently linked by a reaction product of a first functionality coupled to the peptide ligand and a complementary second functionality coupled to the moiety;
• or
• (c) a modified melanocortin 1 receptor (MC1R) peptide ligand-micelle complex, comprising:
• an MC1R peptide ligand;
• and a micelle comprising an inner core, outer core and hydrophilic shell, wherein the MC1R peptide ligand is linked to the shell of the micelle by a linker;
• or
• (d) a pharmaceutical composition comprising a MC1R peptide ligand-micelle complex;
• and a pharmaceutically acceptable carrier, wherein the MC1R peptide ligand-micelle complex comprises an MC1R peptide ligand and a micelle comprising an inner core, outer core and hydrophilic shell, wherein the MC1R peptide ligand is linked to the shell of the micelle by a linker.